蓬子菜中活性成分DXG大鼠血浆蛋白结合率测定OA
Determination of plasma protein binding rate of DXG from Galium verum L.in rat
考察蓬子菜中活性成分DXG在大鼠血浆中的蛋白结合情况.采用平衡透析法测定DXG的大鼠血浆蛋白结合率.建立DXG透析外液和透析内液的HPLC测定方法,求得高、中、低不同质量浓度下,DXG的血浆蛋白结合率分别为29.39%,29.85%,32.94.该方法专属性强,灵敏度高,符合生物样品测定要求要求.DXG的大鼠血浆蛋白结合率较低,且不具有质量浓度依赖性.
To determinate the protein-bound fraction of serum of DXG from Galium verum L.Equilibrium dialysis was used to determine the in vitro binding rate of DXG to rat plasma. A HPLC method for determination of DXG dialysated inside solution and outer solution was established.Plasma protein binding rate of DXG in different concentration was 29.39%, 29.85%, 32.94%, respectively.The methods were specific and sensitive, which can be used in determination of biological…查看全部>>
崔明宇;孙志伟;李艳凤;马英丽
黑龙江中医药大学药学院,哈尔滨150040哈尔滨商业大学药学院,哈尔滨150076黑龙江中医药大学药学院,哈尔滨150040黑龙江中医药大学药学院,哈尔滨150040
医药卫生
蓬子菜DXG血浆蛋白结合率平衡透析法
Galium verum L.DXGplasma protein binding rateequilibrium dialysis
《哈尔滨商业大学学报（自然科学版）》 2015 (6)
678-681,4
国家自然科学基金项目(201281274034) 黑龙江省教育厅科学技术研究项目(12531625)
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