亚热带农业研究2015，Vol.11Issue(4)：222-229,8.
云南八倍体割手密抗逆功能标记遗传多样性探讨
Analysis of genetic diversity among octoploid clones of Saccharum spontaneum L. from Yunnan with functional markers of stress resistance genes
摘要
Abstract
Saccharum spontaneum L. is a wild progenitor and donor species of resistance genes related to environmental stress in sug-arcane breeding programs. In order to know the genetic background of octoploid clones of S.spontaneum and utilize these wild clones more efficiently, five functional PCR markers, namely, DBF/Arb1, DBF/Arb2, Aqua/Arb1, SCB174, and SCB190, which were designed from stress resistance genes DREB, Aquaporin, HSP70 and WRKY1, or paired with arbitrary primer, were used to analyze the genetic diversity of 22 octoploid clones collected from Yunnan. Thirteen key parental lines and 10 major sugarcane cultivars of Yunnan were used as two control populations. The results showed that in total, 113 DNA bands were amplified, of which 106 were polymorphic. There were 17 bands that were population-specific. The mean value of percentage of polymorphic bands ( PPB) was 93.81%. The mean value of polymorphism information content ( PIC) was 91.40%. The octoploid clones showed the highest level of genetic polymorphism according to the PPB and PIC values. A wider range of Jaccard similarity coefficients was found among the oc-toploid clones using functional markers of both drought resistant and heat tolerant genes than among the key parental lines and major cultivars. Population genetic differentiation analysis indicated that the octoploid S. spontaneum clones shared lesser gene exchanges with the two control populations, and only about 30% of the genetic variations were derived among groups. UPGMA clustering results also confirmed that the octoploid S.spontaneum clones exhibited obvious genetic divergence from both control populations by all the five functional markers. Compared with both control populations, the octoploid S.spontaneum clones from Yunnan possess much more abundant genetic variations for heat tolerance and drought resistance genes HSP70, WRKY, DREB and AQP ( especially HSP70 and AQP) . Much of these genetic variations have not been introgressed into the modern sugarcane cultivars. Therefore, the octoploid S. spontaneum clones from Yunnan should be utilized to a greater extent in sugarcane improvement programs.关键词
割手密/八倍体/抗逆性/功能标记/遗传多样性Key words
Saccharum spontaneum L./octoploid/stress resistance/functional marker/genetic diversity分类
农业科学引用本文复制引用
李旭娟,李纯佳,陆鑫,毛钧,刘洪博,林秀琴,徐超华,刘新龙..云南八倍体割手密抗逆功能标记遗传多样性探讨[J].亚热带农业研究,2015,11(4):222-229,8.基金项目
云南省自然科学基金(2011FB120) （2011FB120）
云南省中青年学术技术带头人后备人才(2014HB038) （2014HB038）
云南省应用基础研究计划青年项目(2015FD063). （2015FD063）
