诊断学理论与实践2015，Vol.14Issue(6)：539-544,6.DOI:10.16150/j.1671-2870.2015.06.013
成人慢性免疫性血小板减少性紫癜患者血小板G蛋白偶联受体的研究
Study on expression of G-protein coupled receptors of platelets in adult patients with chronic immune thrombocytopenic purpura
摘要
Abstract
Objective: To investigate the expression of G-protein coupled receptors of platelets in adult patients with chronic immune thrombocytopenic purpura (ITP). Methods: Adenosine diphosphate (ADP) receptor (P2Y1 and P2Y12),α2A-adrenergic receptor (α2A-AR) and thromboxane A2 receptor (TXA2-R) on platelets were detected by flow cytometry, and Gα protein, proteinase activated receptor 1 (PAR-1) and proteinase activated receptor 4 (PAR-4)were analyzed by Western blot. Results: Flow cytometry showed that the mean fluorescence intensities (MFI) of P2Y1, P2Y12, α2A-AR and TXA2-R expressed in platelets of patients with chronic ITP were 31.4±2.2, 29.6±2.1, 25.8±2.9 and 39.8±3.1, respectively, much higher than those from healthy volunteers (P<0.01);MFI of those in healthy volunteers were 7.8±0.8, 7.2±1.3, 9.8± 0.9 and 4.7±0.6, respectively. Western blot analysis showed that the integrated optical density (IOD) of Gαprotein, PAR-1 and PAR-4 expression in platelets of patients with chronic ITP were 1 046.3±159.96, 832.98±98.81 and 1 518.80±272.45, respectively, significantly stronger than those in healthy volunteers (P<0.01); IOD of those in healthy volunteers were 254.49 ±39.51, 203.92 ±27.47 and 431.27 ±41.86, respectively. Conclusions: Expression of G-protein coupled receptors is increased in platelets of patients with chronic ITP, which indicates that platelets of chronic ITP might take part in the complicated biological process by means of the G-protein coupled receptors signal transduction pathway.关键词
慢性免疫性血小板减少性紫癜/血小板/流式细胞仪/G蛋白偶联受体Key words
Chronic immune thrombocytopenic purpura/Platelet/Flow cytometry/G-protein coupled receptors分类
医药卫生引用本文复制引用
邱进锋,王端旭,张森,张摇威,纪少娜,易海宁,赵佳强,尹俊..成人慢性免疫性血小板减少性紫癜患者血小板G蛋白偶联受体的研究[J].诊断学理论与实践,2015,14(6):539-544,6.基金项目
广东省自然科学基金(S2011010004982) （S2011010004982）
广东省大学生创新训练计划(1056012091) （1056012091）
