诊断学理论与实践2015，Vol.14Issue(6)：560-564,5.DOI:10.16150/j.1671-2870.2015.06.017
2013年上海市新发现HIV-1感染者中注射吸毒人群的基因亚型分析
HIV-1 subtype analysis in new HIV-1 infected injection drug users in Shanghai, 2013
摘要
Abstract
Objective: To investigate and analyze the epidemiological feature of human immunodeficiency virus-1 (HIV-1) subtypes infections among injection drug users, newly found and untreated in 2013, Shanghai, for stipulating scientifically and effectively the strategy for intervention. Methods: Seventy-eight cases of new HIV-1 infected injection drug users were sampled and their plasma was collected. HIV-1 pol gene was amplified by reverse transcription polymerase chain reaction(RT-PCR) and then the DNA was sequenced and analyzed by phylogenetic tree, and comprehensive analysis was performed in combination with epidemiological data. Results: Sixty-six samples(84.6%) have been successfully amplified, with the gene sub-type including 47 cases of CRF07_BC(71.2%), 12 cases of CRF01_AE (18.2%), 3 cases of CRF08_BC(4.5%) , 2 cases of sub-type B (3.0%) and 2 cases of subtype C(3.0%). The difference in distribution of CRF07_BC and CRF01_AE showed statistical significant difference by infection source (P=0.02);the 5 kinds of gene subtypes differed between residents and migrates (P<0.01). Conclusions: Genetic diversity of HIV-1 subtype infected injection drug users in Shanghai is complex, and the dominant gene subtype is CRF07_BC. More attention should be paid to this population and regular HIV-1 subtype survilliance is needed.关键词
注射吸毒者/人类免疫缺陷病毒1/亚型/重组体Key words
Injection drug user/Human immunodeficiency virus-1/Subtypes/Recombination分类
临床医学引用本文复制引用
郑敏,郁晓磊,倪莹青,薛以乐,康来仪..2013年上海市新发现HIV-1感染者中注射吸毒人群的基因亚型分析[J].诊断学理论与实践,2015,14(6):560-564,5.基金项目
国家科技部"十二五"重大专项 (2012ZX10001-002-002-012) （2012ZX10001-002-002-012）
