中国药房2015，Vol.26Issue(36)：5131-5133,3.DOI:10.6039/j.issn.1001-0408.2015.36.32
HPLC法测定当归补血配方颗粒中黄芪甲苷的含量
Content Determination of Astragaloside in Danggui Buxue Formula Granule by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for content determination of astragaloside in Danggui buxue formula granule, and investigate the influence of different conditions of dissolution on the content. METHODS:HPLC was performed on the column of Aglient C18 with mobile phase of acetonitrile-water(28:72,V/V)at flow rate of 1.0 ml/min,column temperature was 25 ℃,and the injection volume was 10 μl. RESUITS:The linear range of astragaloside was 2.91-17.46 μg;RSDs of precision,stability were lower than 2%,and reproducibility tests was 3.63%,recovery was 95.76%-98.20%(RSD=1.03%,n=6). The optimized use method was mixing the two granules with 80 ℃ hot water and keeping heat preservation for 5 min successively before taken. CON-CLUSIONS:The method is simple and reproducible,and can be used for the content determination of astragaloside in Danggui bux-ue formula granule.关键词
当归补血配方颗粒/黄芪甲苷/含量测定/高效液相色谱法/冲化条件Key words
Danggui buxue formula granule/Astragaloside/Content determination/HPLC/Melting condition分类
医药卫生引用本文复制引用
李广胜,覃芳,杨季菱,胡文文,张俊丽,张伟欣..HPLC法测定当归补血配方颗粒中黄芪甲苷的含量[J].中国药房,2015,26(36):5131-5133,3.基金项目
河南省中医药科学研究专项课题(No.2014ZY020 40) （No.2014ZY020 40）
