中国药房2015，Vol.26Issue(36)：5133-5135,3.DOI:10.6039/j.issn.1001-0408.2015.36.33
神香草中迷迭香酸和总多糖含量测定方法研究
Stuoly of the Methods of Content Determination of Rosmarinic Acid and Total Polysaccharide in Hyssopus off-cinalis
摘要
Abstract
OBJECTIVE:To establish a method for content determination of rosmarinic acid and total polysaccharide in Hysso-pus. offcinalis. METHODS:HPLC was conducted to determine the content of rosmarinic acid in H. offcinalis. The column was Shim-pack VP-ODS with mobile phase of acetonitrile-0.5% formic(17:83,V/V) at flow rate of 1.0 ml/min,detection wavelength was 330 nm,column temperature was 30 ℃,and the injection volume was 10 μl. UV-visible spectrophotometry was conducted to determine the content of total polysaccharide,detection wavelength was 620 nm. RESULTS:The linear range was 10.86-54.30 μg/ml (r=0.999 8)for rosmarinic acid and 1.996-15.97 μg/ml for total polysaccharide(r=0.999 6);RSDs of precision,stability and re-producibility tests were lower than 1.0% and 2.0%,respectively;recoveries were 98.20%-101.79%(RSD=1.68%,n=6) and 96.62%-101.68%(RSD=1.88%,n=6). CONCLUSIONS:The method is simple and reproducible,and can be used for the con-tents determination of rosmarinic acid and total polysaccharide in H. offcinalis.关键词
高效液相色谱法/神香草/迷迭香酸/总多糖/含量测定Key words
HPLC/Hyssopus offcinalis/Rosmarinic acid/Total polysaccharide/Content determination分类
医药卫生引用本文复制引用
刘桂花,何承辉,刘宣麟,古丽斯坦·阿吾提,邢建国..神香草中迷迭香酸和总多糖含量测定方法研究[J].中国药房,2015,26(36):5133-5135,3.基金项目
新疆维吾尔自治区科技基础条件平台建设项目(No. PT1304) （No. PT1304）
