中国药房2015，Vol.26Issue(36)：5138-5141,4.DOI:10.6039/j.issn.1001-0408.2015.36.35
苍柏祛痛胶囊的质量标准研究
Study on the Quality Standard for Cangbai Qutong Capsule
摘要
Abstract
OBJECTIVE:To establish the quality standard for Cangbai qutong capsule. METHODS:TLC was conducted to iden-tify the Rhizoma atractylodis,Asarum sieboldii,Paeoniae Radix Rubra and Cortex phellodendri;RP-HPLC was conducted to deter-mine the content of berberine hydrochloride. The column was Waters Symmetry-C18 with mobile phase of acetonitrile-0.1% phos-phoric acid (gradient elution) at flow rate of 1.0 ml/min,the detection wavelength was 265 nm,column temperature was 30 ℃, and the injection volume was 10 μl. RESULTS:TLC showed R. atractylodis,A. sieboldii,Paeoniae Radix Rubra and C. phelloden-dri had clear spots and good separation. The linear range of berberine hydrochloride was 21.12-84.48 μg/ml(r=0.999 6);RSDs of pre-cision,stability and reproducibility tests were lower than 3%;recovery was 96.439%-100.950%(RSD=1.34%,n=9). CONCLU-SIONS:The method is simple and reproducible,and can be used for the quality standards of Cangbai qutong capsule.关键词
苍柏祛痛胶囊/盐酸小檗碱/薄层色谱法/反相高效液相色谱法Key words
Cangbai qutong capsule/Berberine hydrochloride/TLC/RP-HPLC分类
医药卫生引用本文复制引用
吴丹枫,贾忠,贾宁,吴晶..苍柏祛痛胶囊的质量标准研究[J].中国药房,2015,26(36):5138-5141,4.基金项目
兰州市人才创新创业项目扶持基金(No.2014-RC-70) （No.2014-RC-70）
