中国药房2015，Vol.26Issue(36)：5141-5143,3.DOI:10.6039/j.issn.1001-0408.2015.36.36
利咽合剂的质量标准研究
Study on the Quality Standard of Liyan Mixture
摘要
Abstract
OBJECTIVE:To establish the quality standard of Liyan mixture. METHODS:TLC was conducted to identify the Scrophularia ningpoensis,Terminalia chebula and Glycyrrhiza uralensis;HPLC was conducted to determine the content of chloro-genic acid. The column was Agilent Eclipse Plus C18 with mobile phase of methanol-0.2% phosphoric acid (18:82,V/V) at flow rate of 1.0 ml/min,the detection wavelength was 327 nm,column temperature was 30 ℃ and the injection volume was 20 μl. RE-SULTS:TLC showed S. ningpoensis,T. chebula and G. uralensis had clear spots and good separation. There was no interference for negative control. The linear range of chlorogenic acid was 0.16-1.6 μg(r=0.999 9);RSDs of precision,stability and reproduc-ibility tests were lower than 1.0%,recovery was 96.9%-101.4%(RSD=1.17%,n=9). CONCLUSIONS:The method is simple and reproducible,and can be used for the quality control of Liyan mixture.关键词
利咽合剂/质量标准/玄参/西青果/甘草/薄层色谱法/绿原酸/高效液相色谱法Key words
Liyan mixture/Quality standard/Scrophularia ningpoensis/Terminalia chebula/Glycyrrhiza uralensis/TLC/Chlorogenic acid/HPLC分类
医药卫生引用本文复制引用
景霞,徐进,孙芳,湛雯..利咽合剂的质量标准研究[J].中国药房,2015,26(36):5141-5143,3.基金项目
南京医科大学科技发展基金项目(No.2012NJMU068) （No.2012NJMU068）
