湖南中医药大学学报2016，Vol.36Issue(1)：10-14,5.DOI:10.3969/j.issn.1674-070X.2016.01.002
丹龙醒脑方对脑缺血再灌注大鼠海马区Notch信号通路相关蛋白表达的影响
Effects of Danlong Xingnao Recipe on Protein Expressions Related to Notch Signaling Pathway in Hipppcampus of Cerebral Ischemia Reperfusion Injury Model Rats
摘要
Abstract
Objective To investigate the effects of Danlong Xingnao recipe (DLXNR) on the expressions of Notch1, Notch2 and Hes1 protein related to the Notch signaling pathway in hippcampus of cerebral ischemia-reperfusion injury model rats. Methods 80 male SD rats were randomly divided into sham-operation group, model group, Danlong Xingnao recipe small-dose group (Dan-small group 7.4 g/kg) and Danlong Xingnao recipe high-dose group (Dan-high group 14.8 g/kg). The local cerebral ischemia /reperfusion rat model was established by intraluminal thread occlusion of the middle cerebral artery. After reperfusion for 7d, the ischemia hippocampus tissues were taken out. The Notch1, Notch2 and Hes1 protein expressions in hippocampal were determined respectively by immunohistochemicstry and Western Blot method. Results Compared with the sham-operation group, the expression of Notch1, Notch2 and Hes1 protein in other groups were increased obviously (P<0.05, P<0.01). Compared with the model group, the expression of Notch1, Notch2 and Hes1 protein in Dan-small group and Dan-high group were increased obviously (P<0.05, P<0.01). Conclusion Danlong Xingnao recipe can upregulate the expressions of Norch1, Notch2 and Hes1 protein, regulate the Notch signal transduction pathway. It may be one of the mechanisms to promote the repair of brain injury.关键词
丹龙醒脑方/脑缺血/海马区/Notch信号通路/Notch1/Notch2/Hes1/丹参/三七/地龙Key words
Danlong Xingnao recipe/brain ischemia/hippcampus/Notch signaling pathway/Notch1/Notch2/Hes1/Salvia miltiorrhiza/Panax notoginseng/lumbricus分类
中医学引用本文复制引用
陈娉婷,周小青,曹泽标,陈昱文,刘旺华,李花..丹龙醒脑方对脑缺血再灌注大鼠海马区Notch信号通路相关蛋白表达的影响[J].湖南中医药大学学报,2016,36(1):10-14,5.基金项目
国家自然科学基金(81373702,81473567,81202632) （81373702,81473567,81202632）
教育部博士点基金(20124323120003) （20124323120003）
湖南省自然科学基金(13JJ3097) （13JJ3097）
湖南省教育厅科研项目(14B134,15K092) （14B134,15K092）
湖南中医药大学研究生创新课题(2015CX15). （2015CX15）
