湖南中医药大学学报2016，Vol.36Issue(1)：15-18,4.DOI:10.3969/j.issn.1674-070X.2016.01.003
知柏地黄汤对UU感染大鼠生精细胞凋亡及凋亡因子Cyt-c、AIF表达的影响
Effects of Zhibai Dihuang Decoction on Spermatogenic Cell Apoptosis of UU Infected Rats and Apoptotic Factors Cyt-c and AIF Expression
摘要
Abstract
Objective To investigate the effect of Zhibai Dihuang Decoction on semen quality, spermatogenic cell apoptosis and apoptosis factors Cyt-c, AIF expression of Ureaplasma urealyticum (UU) infected rats. Methods 45 rats were randomly selected from 60 male SD rats, which were injected with UU by bladder to establish the infective animal models, and the remaining 15 rats were used as normal group. UU infection animal models were randomly divided into model group, azithromycin group (Western medicine group) and Zhibai Dihuang Decoction group (Chinese medicine group), on 10 d after inoculation, the model group and normal group were administrated with saline by gavage, azithromycin group and Zhibai Dihuang Decoction in the treatment group were given corresponding medicine gavage, the animals were executed after continuous intragastric administration for 21 days. The sperm motion parameters, germ cell apoptosis rate and cell apoptosis factor Cytochrome c (Cyt-c) and apoptosis inducing factor (AIF) expression level were detected. Results Compared with the normal group, the sperm quality of the model group was significantly decreased, the positive rate of UU, the apoptosis rate of the cells and the expression of Cyt-c and AIF were significantly increased (P<0.01, P<0.05). Compared with the model group, the sperm quality of Chinese medicine group and Western medicine group were improved, the positive rate of UU, the apoptosis rate, AIF and Cyt-c expression were decreased obviously (P<0.01, P<0.05). Compared with Western medicine group, the sperm quality in Chinese medicine group improved obviously (P<0.05, P<0.01), and lower UU infection rate was not statistically significant (P>0.05). Conclusion Zhibai Dihuang Decoction in improving sperm quality of UU infected rats is better than azithromycin, and its effect on resistant UU infection is equal to azithromycin. The mechanism may be related to decreased expressions of apoptosis factor cytochrome c and AIF.关键词
知柏地黄汤/解脲支原体/生精细胞/细胞色素C/细胞凋亡诱导因子/熟地黄/知母/黄柏Key words
Zhibai Dihuang Decotion/Ureaplasma urealyticum/spermatogenic cell/Cyt-c/AIF/prepared rehmannia root/anemarrhena/phellodendron分类
中医学引用本文复制引用
郭炫佐,何清湖,李迎秋..知柏地黄汤对UU感染大鼠生精细胞凋亡及凋亡因子Cyt-c、AIF表达的影响[J].湖南中医药大学学报,2016,36(1):15-18,4.基金项目
国家自然科学基金(81373641) （81373641）
湖南省科技厅课题资助项目(2011sk3104) （2011sk3104）
长沙市科技局课题资助项目(K1301014-31) （K1301014-31）
湖南省科技计划项目(2012sk3138). （2012sk3138）
