湖南中医药大学学报2016，Vol.36Issue(1)：19-22,4.DOI:10.3969/j.issn.1674-070X.2016.01.004
中药脑泰方对大鼠脑缺血后VEGF和Angiopoietins表达的实验研究
Experimental Research of Naotaifang on Expression of VEGF and Angiopoietins in Rats after Cerebral Ischemia
摘要
Abstract
Objective To investigate Naotaifang on expression of VEGF and angiopoietins in rats after cerebral ischemia, and its roles in angiogenesis. Methods 40 male SD rats were randomly divided into the control group (A), sham-operation group (B),MCAO model group (C),Naotaifang (NTF) group (D). The score of neurological deficiency and volume of cerebral infarction were observed. The protein expression of VEGF, Ang-1 and Ang -2 in cerebral tissues of rats were detected by RT-PCR and Western blot. Results The score of neurological evaluation and TCC staining showed that the cerebral ischemia situation of NTF group have relieved. The expression of TVEGF and Ang-2 were markedly increased, the NTF group is significantly higher than the MCAO group (P<0.05). The expression of Ang-1 in rats after cerebral ischemia was markedly reduced, the NTE group is significantly lower than the model group (P<0.05). Conclusion The coordinating role of VEGF, Ang-1 and Ang-2 expression stimulate angiogenesis after cerebral ischemia. Naotaifang can promote angiogenesis in rats after cerebral ischemia-reperfusion injury.关键词
脑泰方/血管内皮生长因子/血管生成素/脑缺血/黄芪/川芎/地龙Key words
Naotaifang/vascular endothelial growth factors/angiopoietins/cerebral ischemia/astragalus/Ligusticum wal-lichii/earthworm分类
医药卫生引用本文复制引用
宋祯彦,周瑜,王珊珊,龚胜强,刘晓丹,李平,王国佐..中药脑泰方对大鼠脑缺血后VEGF和Angiopoietins表达的实验研究[J].湖南中医药大学学报,2016,36(1):19-22,4.基金项目
国家自然青年科学基金项目(81202794) （81202794）
中西医结合心脑疾病防治湖南省实验室开放基金项目(2014XNFZ05) （2014XNFZ05）
中西医结合防治心脑疾病的相关基础研究湖南高校科技创新团队科研项目(2015CXTD). （2015CXTD）
