湖南中医药大学学报2016，Vol.36Issue(1)：23-26,4.DOI:10.3969/j.issn.1674-070X.2016.01.005
SD大鼠佐剂性关节炎模型的建立与评估
The Establishment and Evaluation of Adjuvant-Induced Arthritis in SD Rats
摘要
Abstract
Objective To explore the best dosage of establishing rheumatoid arthritis model (AIA) rats by caudal injection with different concentration of Mycobacterium tuberculosis H37Ra (Mtb) solution of different groups' SD rats to provide the theory basis for establishing AIA rats methods and its fundamental research. Methods Adopt the Heat-killed Mtb mix with mineral oil, the mixture concentration were 0.625, 1.25, 2.5, 5.0 mg/mL, respectively. the concentration of rats in each group are given the corresponding MT suspension 0.1 mL in the tail root subcutaneously to induce the rheumatoid arthritis. During the experimental period, the rats' body weight was measured at fixed period, the foot paw volume and arthritis were scored, etc, regularly. In the end, the most suitable dosage of inflammatory is selected based on the indexes being synthesized. Results The 62.5 μg can not induce the successful arthritis models and the group of 62.5 μg was removed. The 125 μg, 250 μg and 500 μg groups were all 100%, and there were no significant difference of degree of joint swelling, arthritis score, etc (P>0.05); and the arthritis size and joint swelling degree of rats in 125 μg group were lower than that in 250 μg and 500 μg groups. Conclusion The Mtb induced AIA rat models establishment has a high success rates, and the best inflammatory dosage is probably1.25 mg/mL, 0.1 mL per rat.关键词
SD大鼠/佐剂性关节炎/结核分枝杆菌H37Ra/致炎剂量Key words
SD rats/adjuvant arthritis/Mycobacterium tuberculosis H37Ra/inflammatory dosage分类
医药卫生引用本文复制引用
祁芳,李艳玲,艾坤,蔡雄,李鑫,刘梨,张泓,杨茜芸..SD大鼠佐剂性关节炎模型的建立与评估[J].湖南中医药大学学报,2016,36(1):23-26,4.基金项目
国家自然科学青年基金(81303048) （81303048）
湖南省科技厅科技计划一般项目(2014SK3046) （2014SK3046）
湖南省教育厅科学研究优秀青年项目(15B176) （15B176）
湖南省教育厅科学研究项目(14C0854). （14C0854）
