湖南中医药大学学报2016，Vol.36Issue(1)：27-32,6.DOI:10.3969/j.issn.1674-070X.2016.01.006
回神颗粒减轻中度创伤性脑损伤大鼠脑水肿的疗效机制
The Mechanism Research of Huishen Granule Alleviate Hydrocephalus on Moderate Traumatic Brain Injury Model Rats
摘要
Abstract
Objective To investigate the mechanism of Huishen Granule on hydrocephalus of moderate traumatic brain injury (TBI). Methods Using the equipment of fluid percussion injury (FPI) to establish moderate FPI model in SD rats. The traumatic pressure was (170 ±10) kPa, traumatic time was (20 ±2) ms. The rats were randomly divided into 6 groups: normal group, sham group, model 3 d group, model 7 d group, treatment 3d group, treatment 7 d group. The 3 d and 7 d treatment group were immediately given 2 mL fluid (contain 0.27 g Huishen Granule) by gavage. Then the rats were given by gavage twice for 2 mL every time in morning and evening. The concentration of [Ca2+] in brain cells was examined by using double-beam fluorescent spectrophotometer. The change of NMDAR-1 and AQP-4 in all 6 groups was determined by using fluorescence quantitative polymerization chain reaction (PCR). The TBI pathological change of all 6 groups were observed by using optical microscope and election microscope. Results Compared with the normal group, the [Ca2+] of model 3 d group was increased obviously, the difference was statistically significant (P<0.01). Compared with model 3 d group, the [Ca2+] of treatment 3 d group was decreased obviously, with statistical significance (P<0.01). Compared with the normal group, the NMDAR-1 and AQP-4 expressions of model 3 d group and model 7 d group were increased obviously, which had statistical significance (P<0.01). Compared with model 3 d group, the NMDAR-1 and AQP-4 of treatment 3 d group descended significantly, which had statistic significance (P<0.05). The TBI pathological change of model 3 d group, model 7 d group, treatment 3 d group and treatment 7 d group were observed under optical microscope and election microscope. Compared with the normal and sham-operation groups, it had obvious difference. Compared with model 3 d group and model 7 d group were significantly alleviated. Conclusion Huishen Granule can alleviate traumatic hydrocephalus, maintain the normal structure of neurone, and lessen the TBI. The mechanisms probably were by inhibiting calcium overload and down regulating the NMDAR-1 and AQP-4 expressions.关键词
创伤性脑损伤/脑水肿/细胞内游离钙/N-甲基-D-天门冬氨酸受体1/水通道蛋白4/回神颗粒/人参/石菖蒲/鹿角Key words
traumatic brain injury/hydrocephalus/intracellular free calcium concentrations/NMDAR-1/AQP-4/Huishen Granule/ginseng/acorus gramineus/antlers分类
中医学引用本文复制引用
崔俊波,陈宝贵..回神颗粒减轻中度创伤性脑损伤大鼠脑水肿的疗效机制[J].湖南中医药大学学报,2016,36(1):27-32,6.基金项目
全国名老中医传承工作室建设项目[国中医药人教发(2011)41] （2011）
全国中医药传承博士后资助项目[国中医药人教函(2013)240号] （2013）
天津市"131"创新型人才第一层次人选培养工程[津人才(2013)9]. （2013）
