湖南中医药大学学报2016，Vol.36Issue(1)：33-36,40,5.DOI:10.3969/j.issn.1674-070X.2016.01.007
愈痫灵方对PTZ致痫大鼠脑组织海马CA3区TLR4、NF-kB P65、TNF-α表达的影响
Effects of Yuxianling Decoction on TLR4, NF-kB P65, TNF-α Expressions in Brain Hippocampal CA3 Region of Epileptic Rats Induced by PTZ
摘要
Abstract
Objective To investigate the effect of Yuxianling Decoction on the rats' brain tissue inflammatory factor expression. Methods 120 healthy male SD rats were selected, 10 rats were randomly chosen as a normal control group, 10 rats as the sham-operation group, with normal saline injected in the belly. The other rats were treated with Corazol, and divided the rats that met the standard into Yuxianling group, sodium valproate group, and model group, with 10 rats in each group. The expression of TLR4, NF-kB P65 and TNF-α in brain hippocampal CA3 region were detected by immunohistochemistry. Results Compared with the normal group and sham-operation group, the expression of TLR4, NF-kB P65 and TNF-α of the model group, Yuxianling Decoction group, and the sodium valproate group in hippocampal CA3 region upregulated (P<0.01), while the latter two groups were lower than that of model group (P<0.05), and Yuxianling Decoction group was better than that of sodium valproate group in inhibiting TLR4 and NF -kB P65 expression in hippocampal CA3 region (P<0.05). However, there is no significant differences between the two groups in the expression of TNF-α in brain tissue (P>0.05). Conclusion Yuxianling decoction can inhibit the TLR4, NF-kB P65 and TNF-α expression in hippocampal CA3 region of epileptic rats induced by PTZ and it has certain inhibitory effect on the expression of inflammatory factor in epilepsy.关键词
愈痫灵方/癫痫/炎性反应/肿瘤坏死因子-α/toll样受体4/转录因子P65/川芎/石菖蒲/全蝎Key words
Yuxianling Decoction/epilepsy/inflammatory reaction/TNF-α/TLR4/NF-kB P65/Ligusticum wallichii/acorus gramineus/scorpion分类
中医学引用本文复制引用
张尚波,石学慧,王净净,李智雄,张曦,杨晖,陶琳,曹夏,钟乔青..愈痫灵方对PTZ致痫大鼠脑组织海马CA3区TLR4、NF-kB P65、TNF-α表达的影响[J].湖南中医药大学学报,2016,36(1):33-36,40,5.基金项目
湖南省自然科学基金项目(14JJ2111). （14JJ2111）
