湖南中医药大学学报2016，Vol.36Issue(1)：58-62,5.DOI:10.3969/j.issn.1674-070X.2016.01.015
针刺联合亚低温对脑缺血再灌注损伤大鼠脑组织p-Raf1、p-ERK1/2的影响
The Influence of Acupuncture Combined Hypothermia on p-Raf1 and p-ERK1/2 in Cerebral Ischemia Reperfusion Injury Rats
摘要
Abstract
Objective To observe acupuncture combined hypothermia therapy on cerebral ischemia reperfusion rats nerve function defect scale, cell apoptosis, and the effect of p-Raf1 and p-ERK1/2. Methods Reference ZeaLonga line tied to copy the MCA ischemia model (MCAO) and modified, 50 SD rats were randomly divided into blank group, control group, model group, acupuncture group, acupuncture combined hypothermia group, 10 rats in each group. After 72 h treatment, the nerve function defect scale was observed, apoptosis cells were detected by TUNEL method, ERK1 Raf-1/2 protein expression level in rats brain tissue ischemia side phosphorylation were detected with Western Blot method. Results After building, the model group rats nerve function defect scale increases, the apoptotic cells increased, p-Raf1 and p-ERK1/2 protein expression level increased obviously, compared with the blank group and control group, the difference was statistically significant (P<0.05 or P<0.01). After treatment, the treatment group of nerve function defect grade and phosphorylation, apoptosis cells, p-Raf1 and p-ERK1/2 protein expression levels were significantly lower than that in model group, the difference was statistically significant (P<0.01). Compared with acupuncture group, acupuncture combined hypothermia group neural function defect scale, p-Raf1 and p-ERK1/2 protein expression levels were declined, the difference was statistically significant (P<0.05 or P<0.01). Conclusion The acupuncture and acupuncture combined hypothermia could improve nerve function defect, regulate p-Rafl and reduce the apoptosis, have protection effecton brain, and the combined group showed more stronger effect.关键词
针刺联合亚低温/脑缺血再灌注/p-Raf1/p-ERK1/2/神经功能缺损/细胞凋亡Key words
acupuncture combined hypothermia/cerebral ischemia reperfusion/rats/p-Raf1/p-ERK1/2/nerve func-tion defect/poptotic cells分类
中医学引用本文复制引用
刘琴,林亚平,陈文,肖姮,杨茜芸,陈楚淘,田浩梅..针刺联合亚低温对脑缺血再灌注损伤大鼠脑组织p-Raf1、p-ERK1/2的影响[J].湖南中医药大学学报,2016,36(1):58-62,5.基金项目
国家自然科学基金项目(81303051),湖南省中医药科研计划项目(201471),中医内科学省部共建教育部重点实验室资助(ZYNK201501). （81303051）
