激光生物学报2015，Vol.24Issue(6)：545-550,6.DOI:10.3969/j.issn.1007-7146.2015.06.010
番茄采后寄生菌的分离鉴定及(E)-2-己烯醛抑菌活性研究
Isolation&Identification of Bacteria from Postharvest Tomato and A Study on the Antibacterial Activity of (E)-2-Hexenal
摘要
Abstract
There were many diseases of tomato during postharvest storage and transportation,which seriously affect the quality of tomato.Because of low toxicity and high efficiency,biological control had been used as a new way to control postharvest diseases,became research hotspot.The paper mainly focused on the isolation and purification of the parasitic bacteria on the surface of the mature tomato,the bacterial strains were identified by morphological characteristics,physi-ological and biochemical characteristics,rDNA-ITS sequence analysis and phylogenetic analysis.Antibacterial activity of different concentrations of (E)-2-Hexenal against the bacterial strains were analysed.18 S rDNA genes were amplified by using universal fungal primers,PCR products (about 500 bp) were obtained.After sequencing,the bacterial strains were determined to be Penicillium cyclopium by blasting in NCBI database.According to the growth curve of Penicillium cyclopium,which can be learned that the biomass reached the maximum around 60-72h,the optimum temperature was 25-30 ℃,the optimum pH was 5.0.The antibacterial results showed (E)-2-Hexenal treatment was significantly effec-tives against mycelial growth of Penicillium cyclopium,the MIC was 160 μl/L in growth inhibition assays.关键词
番茄/菌株鉴定/rDNA-ITS序列分析/圆弧青霉/(E)-2-己烯醛/抑菌活性Key words
tomato/strain identification/rDNA-ITS sequential analysis/penicillium cyclopium/(E)-2-Hexenal/an-tibacterial activity分类
生物科学引用本文复制引用
田虎,张继红,孙和龙,高莹,俞可欣,王先友..番茄采后寄生菌的分离鉴定及(E)-2-己烯醛抑菌活性研究[J].激光生物学报,2015,24(6):545-550,6.基金项目
湖南省自然科学基金项目(15JJ6108) （15JJ6108）
湘潭大学博士启动经费(KZ08033) （KZ08033）
