军事医学2015，Vol.39Issue(12)：900-903,4.DOI:10.7644/j.issn.1674-9960.2015.12.003
口服牛骨胶原肽对模拟微重力条件下大鼠股骨微结构的改善效应
Effect of oral administration of bovine collagen peptide on femoral microstructure in rats subjected to simulated microgravity
刘俊丽 1宋淑军 1司少艳 1化楠 1徐冰心 1高姣 1李建忠 1郭燕川2
作者信息
- 1. 解放军306医院特种医学实验研究中心,北京 100101
- 2. 中国科学院化学技术研究所,光化学转换与功能材料重点实验室,北京 100190
- 折叠
摘要
Abstract
Objective To study the bone turnover and femoral microstructural changes induced by oral administration of bovine collagen peptide ( CP) in rats subjected to simulated microgravity.Methods Twenty-four male Sprague-Dawley rats were divided into 3 groups: normal control ( CN) group, simulated microgravity ( SMG) group by tail suspension, SMG with once-daily intragastric administration of 750 mg/kg bovine CP group.CN and SMG groups were given the same dose of normal saline, and the experimental period was 28 d.Serum calcium, phosphorus and alkaline phosphatase(ALP) were measured by an autoanalyzer (Model 7060; Hitachi, Tokyo, Japan), osteocalcin(OC) by radioimmunometric assay and microstructure of the distal end of the right femurs was analyzed by micro CT, and the changes of collagen fibers in the proximal end of tibia were observed with Masson staining.Results SMG+CP produccd osteoprotective effects,as shown by the inhibition of the SMG-induced reductions of serum calcium, ALP and OC levels and improvement of the microarchitecture of the distal femur ( the increase of bone volume and bone trabecular number, inhibition of SMG-induced trabecular alteration and collagen reduction ) .Conclusion Oral administration of bovine CP may produce beneficial efficacy on SMG-induced femoral microstructure changes in rats, which provides a theoretical basis for the prevention and treatment of osteoporosis in a microgravity environment.关键词
胶原肽/模拟微重力/骨代谢/股骨微结构/钙/磷/碱性磷酸酶/骨钙素/骨质疏松Key words
collagen peptide/simulated microgravity/bone turnover/femoral microstructure/calcium/phosphatase/alkaline phosphatase/osteocalcin/osteoporosis分类
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刘俊丽,宋淑军,司少艳,化楠,徐冰心,高姣,李建忠,郭燕川..口服牛骨胶原肽对模拟微重力条件下大鼠股骨微结构的改善效应[J].军事医学,2015,39(12):900-903,4.
