军事医学2015，Vol.39Issue(12)：926-928,933,4.DOI:10.7644/j.issn.1674-9960.2015.12.010
病原微生物实验室实验操作产生气溶胶风险定量研究
Risk quantification of microbiological aerosol generated by experimental operations in pathogenic microbiological labs
摘要
Abstract
Objective To obtain data on risk assessment of microbiological aerosol generated by experimental operations in pathogenic microbiological labs using indicator bacteria and viruses with no infection.Methods The negative pressure lab was used as the experimental environment.Indicator bacteria and viruses (bacteriophages) of a high concentration were used.Many experimental operations and accidental cases were simulated in the lab, including mix-up,accidental fall-off, centrifugal operation,and breaking of centrifugal tubes.The risk of microbiological aerosol generated by these experimental operations was analyzed by quantitative air sampling using Andersen sampler and natural agar sedimentation methods. Results The risk of microbiological aerosol generated by ordinary experimental operations and accidental cases was different.The highest risk of mix-up operation of bacterial aerosol was 1174 colony forming unit(CFU)/m3 and that of viral aerosol was 1058 plaque forming unit ( PFU)/m3 .The highest risk of accidental fall-off of cultivation flasks of bacterial aerosol was 7957 vs 7942 PFU/m3 in viral aerosol.The highest risk of centrifugal tube breaking of bacterial aerosol was 80 CFU/m3 vs 78 PFU/m3 in viral aerosol.Conclusion Experimental operations in pathogenic microbiological lab can generate high risk of bacterial and viral aerosol.Laboratory personnel should abide by the rules and select the appropriate protective equipment.关键词
病原微生物实验室/实验操作/气溶胶风险Key words
pathogenic microbiological laboratory/experimental operations/risk of aerosol分类
医药卫生引用本文复制引用
杜茜,王洪宝,刘克洋,张柯,杨文慧,胡凌飞,王洁,李娜,温占波..病原微生物实验室实验操作产生气溶胶风险定量研究[J].军事医学,2015,39(12):926-928,933,4.基金项目
国家863计划资助项目(2014AA021404) （2014AA021404）
国家科技重大专项资助项目( 2012ZX10004402) （ 2012ZX10004402）
