安徽医科大学学报2016，Vol.51Issue(1)：14-17,4.
促红细胞生成素对脊髓源性神经干细胞分化能力的影响
Effect of erythropoietin on the differentiation of adult spinal cord-derived neural stem cells in vitro
摘要
Abstract
Objective To investigate the promotion of the differentiation of erythropoietin( EPO) on the differentia-tion of the neural stem cells( NSCs) in vitro from spinal cord of SD rats,and to provide experimental basis for the treatment of spinal cord injury by transplantating neural stem cells. Methods The spinal cord was isolated and cul-tured in serum-free suspension, then a differentiation suspension cultured in serum culture medium for 7 days was applied to the NSCs. Nestin was used to detect the NSCs,the differentiation ratio of NSCs into neurons and glia cells was detected by immunofluorescent histochemistry for microtubule-associated protein 2 ( MAP2 ) and glia fibrillary acid protein ( GFAP) . After obtained the third passage ( P3 ) of NSCs, EPO was added to the medium on a final concentration of 10 IU/ml,control group without EPO, the differentiation proportion of MAP2-positive cells to total cells was identified by immunofluorescence staining. Results The results showed that the separated cells from spi-nal cord of SD rats formed neurospheres, in which nestin-positive cells were detected. GFAP and MAP2-positive cells were detected after differentiation culture. The proportion of MAP2-positive cells in the EPO group showed an significant increase compared with the control group ( P<0. 05 ) . Conclusion The results indicate that NSCs can be obtained from the separated spinal cord of SD rats;EPO can increase the differentiation rate of NSCs into neu-rons.关键词
神经干细胞/促红细胞生成素/神经元/分化Key words
neural stem cells/erythropoietin/neuron/differentiation分类
医药卫生引用本文复制引用
李小波,张辉,王少滨,刘杰,余涛,杜怡斌,尹宗生..促红细胞生成素对脊髓源性神经干细胞分化能力的影响[J].安徽医科大学学报,2016,51(1):14-17,4.基金项目
国家自然科学基金(编号:81171173) （编号:81171173）
