北京林业大学学报2016，Vol.38Issue(1)：21-27,7.DOI:10.13332/j.1000-1522.20150196
松材线虫抗逆态基因Bx-DAF6鉴定及基因沉默
Identification and RNAi of a Bursaphelenchus xylophilus dauer formationgene:Bx-DAF6
摘要
Abstract
To explore the molecular mechanism of the Bursaphelenchus xylophilus specialized third-stage dauer larva formation,we studied the Bx-DAF6 gene using the RNAi method.Based on the B.xylophilus genome,Bx-DAF6 was identified by the method of BLAST,and cloned by PCR.The gene function was studied by the suppression of Bx-DAF6 transcript in combination with the RNAi method.The results indicated that the Bx-DAF6 CDS was 2 700 bp,which encodes a protein with a domain homologous to sterol-sensing.The suppression of the Bx-DAF6 transcript by RNAi led to a reduction in the number of specialized third-stage dauer larva formation.Our results indicated that Bx-DAF6 regulated positively the formation of specialized third-stage dauer larvae.关键词
松材线虫/抗逆态幼虫/松树萎蔫病/基因鉴定/基因沉默Key words
Bursaphelenchus xylophilus/dauer larva/pine wilt disease/gene identification/gene suppression分类
农业科技引用本文复制引用
王峰,李丹蕾,马玲,王博文,陈俏丽,张瑞芝,苏丹,康新宇,翟雯..松材线虫抗逆态基因Bx-DAF6鉴定及基因沉默[J].北京林业大学学报,2016,38(1):21-27,7.基金项目
林业公益性行业科研专项(201204501)、黑龙江省自然科学基金项目(ZD201404)、国家自然科学基金项目(31570637)、中央高校基本科研业务费专项资金项目(2572015EY02). （201204501）
