北京林业大学学报2016，Vol.38Issue(1)：36-42,7.DOI:10.13332/j.1000-1522.20150098
转基因白桦不同杂交组合的种子活力测定及外源基因的遗传规律分析
Determination of seed vigor and genetic analysis of foreign genes in different transgenic birch hybrids
摘要
Abstract
Analysis of genetic stability of transgenic lines is an important part of genetic engineering.In this study,we measured growth traits including thousand-seed weight,germination rate,germination potential and vigor index for offspring of transgenic birch .These offspring include families of control-pollination,reciprocal cross and half-sibling families of open pollination.The results showed that all these measured traits were significantly different among these families.Coefficients of variation of these growth traits varied from 41.09% to 73.16% and all the heritabilities were higher than 98.00%.Thousand-seed weight was significantly positively correlated with germination rate,germination potential and vigor index,and the corresponding coefficients of variation were 0.916,0.803 and 0.896,respectively.Interestingly,we found that the foreign genes can be better inherited from female gamete than from androgamete.About 60.00% of offspring from female gamete harbored the foreign genes,while it was only 30.00% of offspring from androgamete.Investigation of the foreign genes of five offspring revealed that four of them had one insertion site,and one had two insertion sites.Chi-square testing of the offspring that had one insertion site indicated that the segregation ratio of gamete was 1:1 .For families which had two insertion sites,the ratio was 3:1.关键词
转基因白桦/种子活力/外源基因检测/传递规律Key words
transgenic birch/seed vigor/detection of foreign gene/inherited rules分类
农业科技引用本文复制引用
陈素素,张嫚嫚,于洪淼,顾宸瑞,魏优,刘宇,王朔,陈肃,刘桂丰..转基因白桦不同杂交组合的种子活力测定及外源基因的遗传规律分析[J].北京林业大学学报,2016,38(1):36-42,7.基金项目
国家级大学生创新创业训练计划项目(201410225058). （201410225058）
