重庆医学Issue(35)：4904-4906,4909,4.DOI:10.3969/j.issn.1671-8348.2015.35.003
原代人子宫内膜细胞分离及培养鉴定
The method of isolation,culture and identification of primary human endometrial cells
摘要
Abstract
Objective To study the isolation method and the culture method of primary human endometrial cells and to iden‐tify the purity and biological activity .Methods We digested human endometrial tissue by collagenase at first ,and then to separate , purify and culture the human glandular stromal cells(ESC) and endometrial epithelial cells(EEC)by differential centrifugation ,dab‐ble screen filtration and adhesion purification technology .Ultimately ,we identified the isolated cells with cytokeratin and vimentin immunocytochemical staining and immunofluorescence method .Results Stromal cells showed a parallel growth .The cells were spindle or polygonal ,and the vimentin antibody showed a positive immunohistochemical staining ,the purity was more than 95% .At the same time ,glandular epithelial cells grew in whorls .The cells were polygonal or tadpole shaped ,and the cytokeratin antibody immunohistochemical staining ,the purity were up to 90% .Conclusion The successful isolation and culture of high quantity ,viabili‐ty and purity by collagenase digestion and dabble screen filter method of endometriall cells make a strong operability .The laboratory which has the basic cell culture conditions can develop the experiment .关键词
子宫内膜/间质细胞/腺上皮细胞/细胞培养Key words
endometrial/stromal cells/epithelial cells/cell incubation分类
医药卫生引用本文复制引用
刘妍,李卫红,韦尉元..原代人子宫内膜细胞分离及培养鉴定[J].重庆医学,2015,(35):4904-4906,4909,4.基金项目
国家自然科学基金资助项目（81260548）。 （）
