重庆医学Issue(35)：4913-4916,4.DOI:10.3969/j.issn.1671-8348.2015.35.006
树突状细胞与调节性T细胞在大鼠动脉粥样硬化中的作用及氟伐他汀的干预效应研究
The effects of fluvastatin on dendritic cells and regulatory T cells in experimental atherosclerosis rat model
摘要
Abstract
Objective To establish experimental atherosclerosis (AS) rat model ,and to investigate the effects of fluvastatin on dendritic cells(DCs) and regulatory T cells in the model .Methods Thirty male Wistar rats were divided into 3 groups:control group(fed with normal diet) ,high‐fat diet group(fed with AS feed) ,fluvastatin group(fed AS diet plus fluvastatin 0 .1 g · kg -1 · d-1 ) .For all the 3 groups ,the samples of aorta were obtained after 20 weeks .The flow cytometry was used to detect the immune‐phenotypic expression of DCs (CD11c ,HLA‐DR) and CD4+ CD25+ FoxP3+ regulatory T cells .Results (1)The numbers of CD11c+ and CD11c+ HLA‐DR+ double positive cells were significantly increased in the high‐fat and fluvastatin group compared with the control group(P<0 .01) ,while fluvastatin treatment reduced the CD11c+ and CD11c+ HLA‐DR+ expression of DCs sig‐nificantly(P<0 .01) .(2)the numbers of CD4+ CD25+ and CD4+CD25+ FoxP3+ regulatory T cells were higher significantly in fluv‐astatin group than the high‐fat group(P<0 .01) .No CD4+ CD25+ and CD4+CD25+ FoxP3+ regulatory T cells was detected in con‐trol group .(3)Correlation analysis between CD11c+ HLA‐DR+DCs and CD4+CD25+ FoxP3+ Treg showed that ,there was a nega‐tive correlation in high‐fat diet group and fluvastain group .Conclusion Fluvastatin could regulate immune‐phenotypic expression of DCs(CD11c ,HLA‐DR) and numbers of CD4+CD25+ FoxP3+ regulatory T cells ,which shows a new immune regulating mechanism of statins for its anti‐atherosclerotic effect .关键词
动脉粥样硬化/树突细胞/调节性T细胞/氟伐他汀/免疫调节Key words
atherosclerosis/dendrtic cells/regulatory T cell/fluvastatin/immunoregulation分类
医药卫生引用本文复制引用
牛川,王清..树突状细胞与调节性T细胞在大鼠动脉粥样硬化中的作用及氟伐他汀的干预效应研究[J].重庆医学,2015,(35):4913-4916,4.基金项目
山东省自然科学基金资助项目（2007ZRB14126）。 （）
