重庆医学Issue(35)：4922-4924,4928,4.DOI:10.3969/j.issn.1671-8348.2015.35.008
shRNA抑制survivin基因表达对脐静脉血管内皮细胞增殖与凋亡的影响
Effects of shRNA-mediated survivin silencing on proliferation and apoptosis of human umbilical vein endothelial cells
摘要
Abstract
Objective To investigate the effect of short hairpin RNA(shRNA) eukaryotic expression vector‐mediated silen‐cing of the survivin‐gene on proliferation and apoptosis of human umbilical vein endothelial cells(HUVEC) .Methods The shRNA vector targeting the survivin gene and negative control vector were transfected into human umbilical vein endothelial cells(HUVEC) incubated with 50 ng/mL of recombinant VEGF in vitro by lipofectamine 2000 .Transfection after 48 h ,the expression of survivin mRNA and protein was detected by quantitative real‐time PCR and Western blot ,respectively .HUVEC proliferation was assayed by four methylthiazolyl tetrazolium(MTT) and cell apoptosis was detected by TUNEL .Results (1)Transfection with survivin‐shR‐NA vector significantly down‐regulated the expression of survivin mRNA and protein as compared with the control group ,after transfection of 48 h(P<0 .05) .(2)After survivin‐shRNA vector transfected ,the proliferation of HUVEC decreased significantly . After transfection 24 ,48 ,72 h ,the growth inhibition rate were (13 .53 ± 3 .91)% ,(38 .97 ± 1 .82)% ,(65 .75 ± 1 .83)% respective‐ly ,at 72 hours after transfection was the most significant .(3)The apoptosis rate of experimental group was (28 .07 ± 1 .71)% , which was higher than the negative control group (11 .45 ± 1 .52)% and blank control group (10 .04 ± 1 .46)% (P<0 .05) .Conclu‐sion The shRNA‐mediated mediated silencing of the survivin‐gene could significantly inhibit proliferation and promote the apopto‐sis of rheumatoid arthritis synovial fibroblasts by regulating survivin expression .关键词
血管内皮生长因子/存活素/shRNA/增殖/细胞凋亡Key words
vascular endothelial growth factor/survivin/short hairpin RNA/proliferation/apoptosis分类
医药卫生引用本文复制引用
马莎,林俊,晋松,李芹,张虹,王静..shRNA抑制survivin基因表达对脐静脉血管内皮细胞增殖与凋亡的影响[J].重庆医学,2015,(35):4922-4924,4928,4.基金项目
云南省科技厅昆明医科大学联合专项基金资助项目（2011FB215）。 （）
