重庆医学Issue(36)：5071-5073,3.DOI:10.3969/j.issn.1671-8348.2015.36.007
高脂高果糖饮食与维生素 D 缺乏对小鼠牙龈菌群影响的实验研究
Experimental research in the effect of high fat high fructopyranose and Vitamin D receptor-knock-out on subgingival bacterial diversity in mice
摘要
Abstract
Objective To detect the oral bacterial diversity in mice under high fat and high fructopyranose diet or/and Vita-min D receptor-knock-out conditions ,in order to study the mechanism between the two conditions and periodontitis .Methods C57BL/6J wild type mice including 20 normal ones and 20 Vitamin D receptor-knock-out ones ,were divided into normal diet and high fat and high fructopyranose diet groups respectively ,10 cases in each group .After feeding 10 weeks ,collecting gingival crevicu-lar fluid from second mandible molar ,analysising the bacterial diversity of the gingival crevicular fluid by using Polymerase chain re-action denaturing gradient gel electrophoresis (PCR-DGGE) method .Collecting specific bands for DNA sequence assay .Results Comparing with normal mice feeding with high fat and high fructopyranose diet ,the gingival index ,periodontal pocket and tooth mobility of Vitamin D receptor-knock-out mice feeding with high fat and high fructopyranose diet had obvious statistical difference (P< 0 .05) ;the distribution of the gingival bacterial diversity between normal mice with normal food and other 3 groups had statis-tical difference(P< 0 .05) .The results of sequence assay showed that the dominan bacteria between normal mice with high fat and high fructopyranose diet and Vitamin D receptor-knock-out mice with high fat and high fructopyranose diet was not the same .Con-clusion It has successfully built a animal model for periodontitis research of high fat and high fructopyranose diet or/and Vitamin D deficient ,the periodontitis will get worse under the effect of the two factor .关键词
牙周病/维生素 D/C57BL /6J 小鼠/高脂高果糖Key words
periodontitis/Vitamin D/C57BL /6J mice/high fat and high fructopyranose分类
医药卫生引用本文复制引用
杨宓,柏雪..高脂高果糖饮食与维生素 D 缺乏对小鼠牙龈菌群影响的实验研究[J].重庆医学,2015,(36):5071-5073,3.基金项目
成都市卫生局青年基金资助项目（2013064）。 （）
