重庆医学Issue(36)：5074-5076,3.DOI:10.3969/j.issn.1671-8348.2015.36.008
糖基因 ST8SIA4在人慢性粒细胞白血病多药耐药中的作用研究
The correlation study of the glycogene ST8SIA4 in the multidrug resistance of human chronic myeloid leukemia
张兆海 1张瑞红2
作者信息
- 1. 辽宁省本溪市金山医院检验科 117000
- 2. 辽宁省本溪钢铁集团总医院手术室 117000
- 折叠
摘要
Abstract
Objective To investigate the differential expression of ST8SIA4 in the both chronic myeloid leukemia(CML) cell lines KCL22 and adriamycin resistant cell lines KCL22/ADR ,peripheral blood mononuclear cells (PBMC) of CML patients ,to clar-ify the correlation of ST8SIA4 and multidrug resistance of CML .Methods Using real-time PCR and Western blot for quantification of ST8SIA4 ,we compared the differential expression of ST8SIA4 in CML cell lines and PBMC of CML patients .The differentially expressed ST8SIA4 gene was silenced ,and the chemosensitivity to anti-tumor drugs was analyzed in vitro .Results ST8SIA4 was differentially expressed in CML cell lines and PBMC of CML patients(P< 0 .05) .The altered level of ST8SIA4 was increased drug-resistant phenotype of KCL22/ADR cells both in vitro and in vivo(P< 0 .05) .Conclusion The expression of ST8SIA4 was different in CML cell lines and PBMC of CML patients ,these characteristic alterations were associated with multidrug resistance in CML .关键词
糖基因 ST8SIA4/多药耐药/粒细胞白血病 ,慢性Key words
glycogene ST8SIA4/multidrug resistance/myeloid leukemia ,chronic分类
医药卫生引用本文复制引用
张兆海,张瑞红..糖基因 ST8SIA4在人慢性粒细胞白血病多药耐药中的作用研究[J].重庆医学,2015,(36):5074-5076,3.
