干旱地区农业研究Issue(6)：161-166,6.DOI:10.7606/j.issn.1000-7601.2015.06.27
外源硫化氢对干旱条件下玉米幼苗抗氧化特性的影响
Effects of exogenous hydrogen sulfide on the antioxidant characteristics of maize seedling under drought
摘要
Abstract
In this paper,10% PEG was used to simulate drought stress,thereby studying the effects of exogenous hydrogen sulfide on the activities of antioxidant enzymes (SOD,POD,CAT and APX),the indexes related with mem-brane lipid peroxidation (MDA and the permeability of cell membrane)in the roots and leaves and the carotenoid content in leaves of maize seedlings under drought stress.The results showed that,compared with drought stress alone,0.01、0.1、0.3、0.6 mmol·L -1 and 1 .0 mmol·L -1 NaHS increased carotenoid content in leaf by 33.3%,63.3%,96.6%, 80.0%,and 26.6%,respectively.Compared with drought stress alone,0.01,0.1,0.3,0.6 mmol·L -1 and 1 .0 mmol·L -1 NaHS increased the POD activity in leaf by 14.5%,36.6%,50.3%,91 .1% and 80.1%.Besides,pre-treatment with 0.01、0.1 mmol·L -1 and 0.3 mmol·L -1 NaHS can significantly improve the activities of SOD and CAT in leaves,the POD activity in root and APX activity in root and leaf.Meanwhile,pretreatment with 0.01,0.1 mmol·L -1 and 0.3 mmol·L -1 NaHS reduced the permeability of cell membrane and the MDA content in root and leaf.0.6 mmol· L -1 NaHS could improve the activities of SOD and CAT in leaves and the activity of POD in root by 7.4%、16.3% and 45 .0%,respectively.Meanwhile,0.6 mmol·L -1 NaHS reduced the permeability of cell membrane of root and the MDA content in leaf by 12.0% and 18.0%,respectively.However,1 .0 mmol·L -1 NaHS reduced the activity of APX in root and leaf by 13.3% and 34.9%,respectively.Due to the decrease in the APX activity,1 .0 mmol·L -1 NaHS increased the permeability of cell membrane of leaf and the MDA content in root by 130.1% and 10.5%,respectively.Collective-ly,these results suggest that pretreatment with low concentrations of hydrogen sulfide can effectively improve the antioxi-dant capacities of maize seedlings under drought stress,especially for 0.3 mmol·L -1 NaHS.关键词
硫化氢/玉米幼苗/干旱胁迫/抗氧化特性Key words
hydrogen sulfide/maize seedling/drought/antioxidant activity分类
农业科技引用本文复制引用
单长卷,周岩,赵元增..外源硫化氢对干旱条件下玉米幼苗抗氧化特性的影响[J].干旱地区农业研究,2015,(6):161-166,6.基金项目
河南省教育厅科学技术研究重点项目 （）
