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卫星搭载苜蓿种子叶片愈伤组织对逆境胁迫的响应

李波 徐婉玉 彭丹 李红

干旱地区农业研究Issue(6):167-171,5.
干旱地区农业研究Issue(6):167-171,5.DOI:10.7606/j.issn.1000-7601.2015.06.28

卫星搭载苜蓿种子叶片愈伤组织对逆境胁迫的响应

Response to abiotic stresses of leaf callus of alfalfa seed carried by satellite

李波 1徐婉玉 1彭丹 1李红2

作者信息

  • 1. 齐齐哈尔大学生命科学与农林学院,黑龙江 齐齐哈尔 161006
  • 2. 黑龙江省畜牧研究所,黑龙江 齐齐哈尔 161000
  • 折叠

摘要

Abstract

To study the influence of space environment on the leaf callus of alfalfa seed carried by satellite,the cal-lus of alfalfa under different stress (NaCl and PEG and temperature stress)were studied,and the five physiological pa-rameters including proline,soluble sugar,soluble protein,relative conductivity and MDA content were determined to e-valuate the effect of satellite carrying on callus resistance.The results showed that the survival rates of no-carrying materi-al were 2.68%,3.8%,43.68%,and 35 .42% for NaCl (2%),PEG (30%),high temperature (40℃,6d),and low temperature (0℃,8d)stress,being significantly different with those of the control.Satellite-carrying resulted in higher proline,soluble sugar,protein contents,by an increase of 55 .6%、34.71%,and 40.67%,respectively.By contrast,the relative conductivity and MDA content were reduced by 48.37% and 39.47%.The degree of resistance of satellite carrying alfalfa callus was in the order of drought,salt,low temperature,and high temperature.

关键词

苜蓿/愈伤组织/卫星搭载/生理指标/抗逆

Key words

alfalfa/callus/space flight/physiological parameters/resistance

分类

农业科技

引用本文复制引用

李波,徐婉玉,彭丹,李红..卫星搭载苜蓿种子叶片愈伤组织对逆境胁迫的响应[J].干旱地区农业研究,2015,(6):167-171,5.

基金项目

黑龙江省教育厅科学技术研究资助项目 ()

干旱地区农业研究

OACSCDCSTPCD

1000-7601

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