干旱地区农业研究Issue(6):167-171,5.DOI:10.7606/j.issn.1000-7601.2015.06.28
卫星搭载苜蓿种子叶片愈伤组织对逆境胁迫的响应
Response to abiotic stresses of leaf callus of alfalfa seed carried by satellite
摘要
Abstract
To study the influence of space environment on the leaf callus of alfalfa seed carried by satellite,the cal-lus of alfalfa under different stress (NaCl and PEG and temperature stress)were studied,and the five physiological pa-rameters including proline,soluble sugar,soluble protein,relative conductivity and MDA content were determined to e-valuate the effect of satellite carrying on callus resistance.The results showed that the survival rates of no-carrying materi-al were 2.68%,3.8%,43.68%,and 35 .42% for NaCl (2%),PEG (30%),high temperature (40℃,6d),and low temperature (0℃,8d)stress,being significantly different with those of the control.Satellite-carrying resulted in higher proline,soluble sugar,protein contents,by an increase of 55 .6%、34.71%,and 40.67%,respectively.By contrast,the relative conductivity and MDA content were reduced by 48.37% and 39.47%.The degree of resistance of satellite carrying alfalfa callus was in the order of drought,salt,low temperature,and high temperature.关键词
苜蓿/愈伤组织/卫星搭载/生理指标/抗逆Key words
alfalfa/callus/space flight/physiological parameters/resistance分类
农业科技引用本文复制引用
李波,徐婉玉,彭丹,李红..卫星搭载苜蓿种子叶片愈伤组织对逆境胁迫的响应[J].干旱地区农业研究,2015,(6):167-171,5.基金项目
黑龙江省教育厅科学技术研究资助项目 ()