河南农业科学2016，Vol.45Issue(1)：127-130,4.DOI:10.15933/j.cnki.1004-3268.2016.01.028
豫西地区禽源大肠杆菌的分离鉴定与耐药性分析
Isolation,Identification and Drug Resistance Analysis of Escherichia coli from Chickens in Western Henan Province
摘要
Abstract
In order to investigate the resistance of Escherichia coli isolated from chickens in western Henan province,a total of 130 samples of suspect of Escherichia coli were examined for the presence of Escherichia coli species. All bacteria were isolated by a conventional method,identificated via biochemical testing,PCR,serotyping and antimicrobial susceptibility test. 98 strains of E. coli were separated and 86 strains were confirmed as belonging to 14 serum types. The chief serum types were O14 ,O141 ,O78 and O88 with the percentage of 20. 93%,16. 28%,13. 95% and 9. 30% respectively. The isolates were evaluated for antimicrobial sensitivity by K-B disc diffusion method against 20 antimicrobial drugs. Among E. coli isolates,95. 92%,83. 67%,81. 63%,77. 55%,69. 39% were resistant to sulfamethoxazole,tetracycline, ampicillin,nilidixic acid and enrofloxacin, respectively. Almost isolates of E. coli were susceptible to polymyxin B,imipenem. Most of isolates were multiple resistant strains. 77. 55% of the isolates (76) were resistant to 9—14 antimicrobial agents. This study revealed that the drug resistance of all isolates was a severe problem in western Henan province,which would seriously affect diagnosis and prevention of E. coli in poultry.关键词
大肠杆菌/分离鉴定/药敏试验/耐药性分析Key words
E. coli/isolation and identification/antimicrobial susceptibility testing/antimicrobial resis-tance analysis分类
农业科技引用本文复制引用
毛福超,郁川,韩璐,韩于佩,刘文慧,刘静..豫西地区禽源大肠杆菌的分离鉴定与耐药性分析[J].河南农业科学,2016,45(1):127-130,4.基金项目
国家自然科学基金项目(31302059) （31302059）
河南科技大学SRTP项目(2015143) （2015143）
河南科技大学博士启动基金项目(4025-13480064) （4025-13480064）
