吉林农业大学学报Issue(6)：698-701,713,5.DOI:10.13327/j.jjlau.2015.2870
线粒体途径参与FTY720诱导白血病细胞凋亡的研究∗
Involvement of Mitochondrial Pathway in FTY720-induced Leukocyte Apoptosis
摘要
Abstract
The studyaims to clarify the relationship between mitochondrial pathway and apoptosis of K562 cells induced by FTY720. Human leukemia ( K562 cells) were cultured in vitro and treated with different concentrations of FTY720 for 24 h, and then survival rate of K562 cells was detected by trypan blue staining method; apoptosis and mitochondrial function were tested by flow cytometry and expression of Bcl⁃2 and Bax related to mitochondria was detected by Western blotting. K562 cells induced by FTY720 for 48 h were collected and cell cycle was measured by flow cytometry. Through the study, we found that with the increase of concentration of FTY720, survival rate of K562 cells was decreased, apoptosis was increased, mitochondrial membrane potential was de⁃creased , and expression of apoptosis promoting protein Bax was significantly increased . Cell cycle a⁃nalysis showed that percentage of SubG1 phase cells was increased while S phase and phase G2M cells were decreased. The study showed that mitochondrial pathwaywas were involved in apoptosis of K562 cells induced by FTY720.关键词
线粒体/FTY720/细胞凋亡Key words
mitochondria/FTY720/cell apoptosis分类
医药卫生引用本文复制引用
张宸豪,陈为,李瑶,李妍..线粒体途径参与FTY720诱导白血病细胞凋亡的研究∗[J].吉林农业大学学报,2015,(6):698-701,713,5.基金项目
国家自然科学基金项目（82102953），吉林省科技厅中青年科技创新领军人才及团队项目（20130521018JH），吉林省科技厅重大科技攻关项目（20140203012YY），吉林省卫生厅项目（2013 Z091），吉林省教育厅项目 （）
