吉林农业大学学报Issue(6)：719-725,7.DOI:10.13327/j.jjlau.2015.2650
重组蓖麻毒素A链与天然蓖麻毒素毒性与免疫效果比较∗
Comparative Study of Toxicity and Immunity of Recombinant Ricin A Chain and Natural Ricin
摘要
Abstract
The purpose of this research is to compare toxicity and immune effect of recombinant ricin A chain ( r⁃RTA) , recombinant mutant ricin A chain ( m⁃RTA) and natural ricin ( n⁃RT) . In cell test, toxicity of m⁃RTA and r⁃RTA is 2 700 times lower than n⁃RT. After Intraperitoneal injection immunization without adjuvant r⁃RTA, m⁃RTA and n⁃RT, all mice lost weight during the immune prograss, and compared to the group immunized with r⁃RTA and n⁃RT, mice in the group immu⁃nized with m⁃RTA had the smallest weight change. After four times of immunization,serum antibody titers of mice could reach above 10-4 , immunization of mice with 6 mg/kg m⁃RTA could provide full protection for 20 × LD50 dose of ricin infected. The results show that r⁃RTA, m⁃RTA and n⁃RT are all toxic to mice to some extent, and mutant m⁃RTA irritation can provide better protection to mice,which will provide vital data and theoretical supports for the development of RTA⁃based vaccine.关键词
重组/突变/蓖麻毒素/免疫效果Key words
recombinant/mutant/ricin/immune effect分类
农业科技引用本文复制引用
曲英龙,钱军,郭振东,赵思言,钱爱东,刘林娜..重组蓖麻毒素A链与天然蓖麻毒素毒性与免疫效果比较∗[J].吉林农业大学学报,2015,(6):719-725,7.基金项目
国家自然科学基金项目 （）
