检验医学与临床2016，Vol.13Issue(2)：145-146,2.DOI:10.3969/j.issn.1672-9455.2016.02.001
白细胞介素-35 对乙型肝炎病毒复制的影响研究
Study on influence of interleukin-35 on replication of hepatitis B virus
摘要
Abstract
Objective To explore the influence of interleukin-35(IL-35) on the replication of hepatitis B viru (HBV) .Methods HBV infectious clone pHBV1 .3 and its promoter pHBV-Luc were transfected into HepG2 cells respectively and different concentrations of IL-35 were added .The HBsAg and HBeAg levels in the supernatants were measured by adopting enzyme linked immunosorbent assay (ELISA) ,and the covalently closed circular DNA (cccDNA) levels were measured by real-time PCR ,and the HBV promoter activity was measured by the luminometer fluorescence detector .Results The levels of HBsAg and HBeAg ,and cccDNA copy number were inhibited by IL-35 and the HBV promoter activity was down-regulated .Conclusion IL-35 can inhibit the replication of HBV in HepG2 cells .关键词
乙型肝炎病毒/白细胞介素-35/酶联免疫吸附试验/复制Key words
HBV/IL-35/ELISA/replication引用本文复制引用
祝成亮,李艳,袁丽..白细胞介素-35 对乙型肝炎病毒复制的影响研究[J].检验医学与临床,2016,13(2):145-146,2.基金项目
国家自然科学基金资助项目(81101485 ) （81101485 ）
国家临床重点专科专项经费资助项目(2010305 ) （2010305 ）
湖北省自然科学基金资助项目(ZRY2014000315). （ZRY2014000315）
