检验医学与临床2016，Vol.13Issue(2)：147-149,152,4.DOI:10.3969/j.issn.1672-9455.2016.02.002
β-连环蛋白在铜绿假单胞菌感染时的调节作用
Regulating role of β-catenin in Pseudomonas aeruginosa infection
摘要
Abstract
Objective To explore the regulating role ofβ-catenin in bacterial infection caused inflammation re-action and bacterial clearance after Pseudomonas aeruginosa infection .Methods Western-blot and immunofluores-cence assay were used to determine theβ-catenin expression in macrophage-like RAW264 .7 cells and neutrophils be-fore and after Pseudomonas aeruginosa infection;the upregulating effect ofβ-catenin after lentivirus infection was de-tected by Western-blot ;the pro-inflammatory cytokine expression was examined by using real-time PCR(RT-PCR);the bacterial load was assessed by using the plate count assay .Results Theβ-catenin protein expression in Pseudo-monas aeruginosa infected macrophages and neutrophils showed the transient upregulation and then was gradually de-creased .β-catenin suppressed the expression of pro-inflammatory cytokines and promoted bacterial clearance in Pseudomonas aeruginosa infected macrophages and neutrophils .Conclusion β-catenin inhibits the inflammation reac-tion and enhances the bacterial clearance in Pseudomonas aeruginosa infection .关键词
β-连环蛋白/铜绿假单胞菌/炎性反应/细菌清除Key words
β-catenin/Pseudomonas aeruginosa/inflammation reaction/bacterial clearance引用本文复制引用
陈康,傅强,阚丽娟,王霞,孙世珺,张秀明..β-连环蛋白在铜绿假单胞菌感染时的调节作用[J].检验医学与临床,2016,13(2):147-149,152,4.基金项目
国家自然科学基金青年基金资助项目(81401645) （81401645）
广东省医学科研基金资助项目(B2014447) （B2014447）
广东省中山市科技计划资助项目(2014A1FC098). （2014A1FC098）
