检验医学与临床2016，Vol.13Issue(2)：153-156,159,5.DOI:10.3969/j.issn.1672-9455.2016.02.004
长链非编码 RN A M EG3 抑制食管癌细胞增殖和侵袭作用研究
Study on effect of long non-coding RNA MEG3 for inhibiting proliferation and invasion of esophageal carcinoma cells
摘要
Abstract
Objective To investigate the regulating effect of long non-coding RNAs (lncRNAs) MEG3 on the biological behaviors of proliferation and invasion in esophageal cancer cells .Methods 42 samples of tumor tissue (tumor group) in the operative patients with esophageal cancer and 42 samples of paracancerous normal esophageal tissue(control group) were collected and the expression level of MEG3 was detected by using real-time quantitative PCR(RT-PCR) .The expression differences of MEG3 were compared between the tumor tissue and the normal tis-sue .Polymine(PEI) and liposome were adopted to conduct the over-expression or the interference elimination tests of MEG3 ;MTT assay and Transwell chamber were performed to detect the proliferation and invasion activities of KYSE30 cell in each group .Results The RT-PCR results revealed that compared with the normal tissue ,lncRNA MEG3 showed the low expression in esophageal cancer tissue .Moreover ,over-expression of MEG3 inhibited KYSE30 cell proliferation ,however ,after eliminating MEG3 ,the proliferation and invasion of esophageal cancer cells were in-creased .Conclusion LncRNA MEG3 participates in the development and progression of esophageal cancer and plays a key role as a tumor inhibiting gene in esophageal cancer .关键词
MEG3/长链非编码RNA/食管癌/增殖/侵袭Key words
MEG3/long non-coding RNA/esophageal cancer/proliferation/invasive引用本文复制引用
冯晓倩,向代军,王海..长链非编码 RN A M EG3 抑制食管癌细胞增殖和侵袭作用研究[J].检验医学与临床,2016,13(2):153-156,159,5.基金项目
国家科技基础条件资助项目(2005DKA32403-31). （2005DKA32403-31）
