检验医学与临床2016，Vol.13Issue(2)：160-162,165,4.DOI:10.3969/j.issn.1672-9455.2016.02.006
4种固定破膜试剂盒检测人调节性 T 淋巴细胞的结果比较
Results comparison of four kinds of fixation permeabilization reagent kit for detecting regulatory T lymphocytes
摘要
Abstract
Objective To compare the effects of four kinds of fixation permeabilization reagent kit for detec-ting regulatory T(Treg) lymphocytes .Methods Three kinds of antibody of CD4 ,CD25 and Foxp3 were selected to establish the flow Treg lymphocytes detection scheme .The fresh peripheral blood samples from 10 healthy individu-als were collected for separating mononuclear cells .The membrane surface antibodies CD4 and CD25 were firstly la-beled .T hen the four kinds of fixation permeabilization reagent kit ,BD Pharmingen ,eBioscience ,Invitrogen and Beck-man Coulter ,were used for performing the fixation and permeabilization on cells .Finally Foxp3 antibody was labelled and detected on machine .Results Compared with the unfixation pemeabilization corresponding samples ,the four kinds of reagent kit had a obvious impact on the morphology of the cells ,but no obvious effect on the expression of CD4 and and CD25 .The detected proportions (CD4+ CD25+ Foxp3+ ) were(8 .22 ± 2 .75)% for the eBioscienc fixa-tion permeabilization reagent kit ,(0 .54 ± 0 .48)% for the Beckman Coulter reagent kit ,(0 .69 ± 0 .33)% for the BD Pharmingen reagent kit and (0 .41 ± 0 .24)% for the Invitrogen reagent kit .Conclusion The four kinds of reagent kit have similar fixation effect .The eBioscience fixation permeabilization reagent kit has better detection effect and is more suitable for detecting Treg lymphocytes .关键词
调节性T淋巴细胞/叉状头转录因子3/固定破膜Key words
regulatory T lymphocytes/Foxp3/fixation permeabilization引用本文复制引用
王方芳,黄巧容,张传芬,朱国念,孟文彤..4种固定破膜试剂盒检测人调节性 T 淋巴细胞的结果比较[J].检验医学与临床,2016,13(2):160-162,165,4.基金项目
四川省卫生和计划生育委员会科研课题(JH2014067). （JH2014067）
