检验医学与临床2016，Vol.13Issue(2)：166-168,3.DOI:10.3969/j.issn.1672-9455.2016.02.008
卵巢癌干细胞的分离 、鉴定及初步生物学功能研究
Study on separation ,identification and preliminary biological function of ovarian cancer stem cells
摘要
Abstract
Objective To isolate the CD133CD117 phenotype ovarian cancer stem cells from the SKOV3 cell line of human ovarian carcinoma ,and to investigate its related biological function .Methods The CD133CD117 cancer cells were sorted through MACS technology ,then the plates ,soft agar colony formation assays ,serum-free culture and tumorigenicity test were carried out to investigate its preliminary function;RT-PCR was used to detect cancer cell Wnt-2 gene expression .Results The cloning formation capability of CD133CD117 cancer cells in petri dishes ,soft agar and serum-free medium was stronger than that in CD133-CD117- cancer cells ;meanwhile showing stronger tu-morigenicity ;the expression levels of Wnt-2 gene were different among different phenotypes of cancer cells .Conclu-sion Stem cell-like cancer cells exist in ovarian cancer cells ,sorted CD133CD117 cancer cells from ovarian cancer cells by MACS have the properties of stem cells ,which provides the scientific basis for the study of biological charac-teristics and targeted therapy of ovarian cancer stem cells .关键词
卵巢癌干细胞/免疫磁珠分选/Wnt-2基因Key words
ovarian cancer stem cells/magnetic activated cell sorting/Wnt-2引用本文复制引用
刘春生,浦春,曹明刚,张鹏..卵巢癌干细胞的分离 、鉴定及初步生物学功能研究[J].检验医学与临床,2016,13(2):166-168,3.基金项目
安徽省教育厅高校自然科学基金资助项目(KJ2013B323) （KJ2013B323）
皖南医学院中青年基金资助项目(WF2012F27). （WF2012F27）
