临床口腔医学杂志Issue(12)：721-724,4.DOI:10.3969/j.issn.1003-1634.2015.12.005
放射线对口腔上皮细胞抗白色念珠菌感染能力的研究
Influence of the radioactive rays on resistance to infection of oral epithelial cell to Candida albicans
王静 1孙海燕 2唐青 3陈芳艳 4刘继光1
作者信息
- 1. 佳木斯大学 黑龙江 佳木斯 154002
- 2. 解放军第307医院口腔科 北京 100071
- 3. 四川大学生命科学学院 四川 成都 610065
- 4. 解放军疾病预防控制所 北京 100071
- 折叠
摘要
Abstract
Objective:To discuss the Influence of X-ray on resistance to infection of oral epithelial cells to Candida albicans. Method:The oral epithelial cells were irradiated with 1,3,5,7 and 9 Gy. Oral epithelial cell viability was measured by MTT assay. The number of Candida albicans cells that were invading oral epithelial cells was determined using a protocol called nystatin protection assay. Adherence assays were performed with the oral epithelial cell line according to a previously described protocol calcofluor white staining assay. Result:MTT assay of the oral epithelial cells showed a decrease of cell viability directly with an increase in irradiation dose (P<0.05). Compared with the control group,the group which irradiated with 9 Gy showed significant reduction in invasion(P <0.05),but had an increased ability in adhesion(P <0.05). Conclusion:MTT assay of the oral epithelial cells showed a decrease of cell viability as the irradiation dose increased gradually. The cell viability of the oral epithelial cells statistically decreased when the irradiated dose was 5 Gy (P<0.05). X-ray had a significant influence on resisting to infection of oral epithelial cell to Candida albicans ,but the specific impact of the mechanism needs a further study.关键词
白色念珠菌/口腔上皮细胞/X线/MTT/侵染率/粘附率Key words
candida albicans/oral epithelial cell/X-ray/MTT/adhesion/invasion分类
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王静,孙海燕,唐青,陈芳艳,刘继光..放射线对口腔上皮细胞抗白色念珠菌感染能力的研究[J].临床口腔医学杂志,2015,(12):721-724,4.
