临床口腔医学杂志Issue(12)：728-731,4.DOI:10.3969/j.issn.1003-1634.2015.12.007
抗真菌治疗和年龄因素对OLP角质形成细胞体外培养成功率的影响
The influence of anti-fungus treatments and age factors to in vitro culture system for keratinocytes of OLP
摘要
Abstract
Objective:The aim of this study was to establish a stable in vitro culture system for keratinocytes obtained from the lesion of patients with oral lichen planus (OLP) and evaluate the successful rate of anti-fungus treatments and age factors. Method:Reticular OLP patients are divided into groups by anti-fungus treatments and age. OLP mucosa (histopathologically confirmed) was collected and cells isolated using the cold enzyme digestion method. Primary culture and serial passage were performed on oral keratinocyte medium. The successful rates are anaysised by Fisher's exact test. Mor-phological changes of cells were evaluated via inverted phase contrast microscopy. Result:Cells from OLP patients who ac-cepted anti-fungus treatments or not older than 40 years have better rate and the results showed significant difference (P*=0.03,P**=0.015). Conclusion:This study proved the tissues which taken from younger patients or patients under anti-fun-gus treatments have a higher successfulrate to be cultured,which improved the establishment of human OLP cell model in vitro.关键词
口腔扁平苔藓/角质形成细胞/抗真菌治疗/体外培养Key words
oral lichen planus/keratinocytes/anti-fungus treatments/culture in vitro分类
医药卫生引用本文复制引用
曹天翼,陈福祥,唐国瑶,王万春..抗真菌治疗和年龄因素对OLP角质形成细胞体外培养成功率的影响[J].临床口腔医学杂志,2015,(12):728-731,4.基金项目
国家自然科学基金（81170967）；2013～2014国家临床重点专科建设项目资助 （）
