河南科技大学学报（医学版）Issue(4)：241-243,3.
昆明地区碳青霉烯耐药肠杆菌科细菌耐药性分析及基因型检测
Drug Resistance and Genotype Analysis of Carbapenem-resistant Enterobacteriaceae
摘要
Abstract
Objective To investigate the drug resistance and genotype of carbapenem-resistant Enterobacteriaceae (CRE)in Kunming. Methods 77 CRE isolates were collected from January 2009 to December 2012.Their carbapenemase phenotypes were detected by modified Hodge test and screened metallo-B-lactamase positive strains by EDTA disc synergy test.The blaNDM-1 ,blaKPC,blaIMP and blaVIM genes of carbapenemase were detected by PCR,then the obtained PCR products were performed DNA sequencing. Results 75.32 percentage of CRE isolates were identified to be Klebsiella pneumoniae,and most of CRE are multi-drug resistant bacteria.The positive results of CRE isolates in the modified Hodge test and EDTA disc synergy test were 55 and 15 isolates respectively.The blaKPC gene was identified from 45 isolates by PCR,9 blaNDM isolates and 4 blaIMP gene isolates were detected.IMP-4 and KPC-2 isolates was identified by DNA sequencing.The target gene of 18 CRE isolates were not detected. Conclusion The KPC-2 and NDM-1 were the major genotypes of carbapenemase in Kunming hospital. It was important that drug resistance surveillance of CRE isolates.Target gene of 18 isolates were not detected,so their drug resistance mechanism should be further experimental study.关键词
肠杆菌科细菌/碳青霉烯酶/耐药性/基因型Key words
enterobacteriaceae/carbapenemase/drug resistance/genotype分类
医药卫生引用本文复制引用
胡莹,邰文琳,马润,王卓,番寿蕊,王玉明..昆明地区碳青霉烯耐药肠杆菌科细菌耐药性分析及基因型检测[J].河南科技大学学报（医学版）,2015,(4):241-243,3.基金项目
云南省应用基础研究面上项目 （）
