河南科技大学学报（医学版）Issue(4)：244-247,251,5.
bFGF 在百草枯诱导大鼠肺纤维化过程中的表达
Expression of bFGF in Process of Pulmonary Fibrosis Induced by Paraquat in Rats
摘要
Abstract
Objective To establish the model of pulmonary fibrosis by paraquat (PQ ) in rats and investigate the expression of basic fibroblast growth factor(bFGF)in the pulmonary fibrosis induced by PQ. Methods 45 healthy male SD rats were divided into 1 d,3 d,5 d,7 d,10 d,14 d,21 d,28 d,and blank control group with 5 rats in each group.Rats in the experimental group were intraperitoneally injected with 10 mg·kg -1 PQ solution,and the control group was injected isotonic saline solution.Rats in the experimental group were sacrificed at observation time,and the control group was fed 28 d.The pulmonary morphology changes,hematoxylin eosin staining (HE ) and Masson staining of lung tissues were observed,and the immunohistochemistry method was used to detect the expression of bFGF protein in lung tissues of rats. Results After exposure of PQ the rat appears abnormal behavior in experimental group.The neutrophil infiltration were observed in rats after exposure of PQ 3 d,and proliferation of fibroblasts after 5 d,and the irregular collagen fibers were obviously increased surrounding the alveolar after 14 d.Pulmonary fibrosis was formed obviously after 28 d.In the experimental group,the positive expression of bFGF was found in the cytoplasm of inflammatory cell,bronchial mucosal epithelium and alveolar wall.Furthermore,the positive expression of bFGF was increased with exposure time until to 28 d,and then that was slightly decreased. Conclusion The typical pulmonary fibrosis was induced by PQ poisoning,and the degree of pulmonary fibrosis was continuously aggravated with exposure time. The expression of bFGF was increased with exposure time of PQ until to 28 d, and then that was decreased. Its mechanism need to be further studied.关键词
百草枯/肺纤维化/大鼠/bFGFKey words
paraquat/pulmonary fibrosis/rat/bFGF分类
医药卫生引用本文复制引用
徐宏业,乔博亚,张杰,鲍志宇,秦豪杰,莫耀南..bFGF 在百草枯诱导大鼠肺纤维化过程中的表达[J].河南科技大学学报（医学版）,2015,(4):244-247,251,5.基金项目
河南省教育厅基础研究项目（14B310001）河南科技大学大学生研究训练计划项目 （）
