河南科技大学学报（医学版）Issue(4)：248-251,4.
慢病毒介导稳定表达 miR-183的人卵巢癌 skov3细胞系
Establishment of Human Ovarian Cancer Skov3 Cell Line Stably Expressing miR-183 by Lentiviral System
陈亚伟 1樊冬梅 1刘刚 1田晓予1
作者信息
- 1. 河南科技大学第一附属医院，河南洛阳 471003
- 折叠
摘要
Abstract
Objective To establish human ovarian cancer skov3 cell line stably expressing miR-183. Methods The gene of Pre-miR-183,has-miR-183 inhibitor were cloned into eukaryotic expression vector, respectively,yielding pEZX-MR03-183 mimics,pEZX-AM03-183 inhibitor.The pEZX-MR03-183 mimics, pEZX-AM03-183 inhibitor were transfected into 293T cells for acking lentivirus,and the lentivirus transfected in human ovarian cancer skov3 cells.Screening with puromycin and hygromycin B,there got the stable transfection skov3 cell line,and through RT-PCR to verify the expression of miR-183 at transcription level. Results The monoclonal skov3 cell line with stable high and low expression of miR-183 were successfully isolated and cultured. Conclusion The study successfully establish the stably transfected skov3 cell lines of miR-183 that could be used to be an effective cell model to research the biological functions of miR-183.关键词
慢病毒/skov3 细胞/miR-183/卵巢瘤Key words
lentivirus/skov3 cell/miR-183/ovarian cancer分类
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陈亚伟,樊冬梅,刘刚,田晓予..慢病毒介导稳定表达 miR-183的人卵巢癌 skov3细胞系[J].河南科技大学学报（医学版）,2015,(4):248-251,4.
