农业环境科学学报Issue(12)：2280-2286,7.DOI:10.11654/jaes.2015.12.005
BDE-47对斑马鱼胚胎氧化应激与DNA损伤的毒性研究
Oxidative Stress and DNA Damage in Zebrafish Embryos Exposed to BDE-47
摘要
Abstract
Polybrominated diphenyl ethers(PBDEs), widely used flame retardants, have been detected in environment, biota, and even hu-man blood serum. Currently, little is known about their toxicity to fish. This experiment was designed to explore the oxidative stress and DNA damage of 2,2',4,4'-tetrabromodi-phenyl ether(BDE-47)to zebrafish embryos. Superoxide dismutase(SOD), catalase(CAT), total glutathione(GSH), malondialdehyde(MDA), and relative mRNA expression levels of genes(Sod1、Ucp-2、Gstp2 and Nqo1)were assessed in zebrafish embryos exposed to 1, 5, 10, and 50μg·L-1 of BDE-47 for 96 hours. The single cell gel electrophoresis was performed to ob-serve the DNA damage caused by BDE-47. Compared with control, reactive oxygen species(ROS)and MDA were increased by BDE-47 treatments in a dose-effect manner. Exposure to 50μg·L-1 of BDE-47 for 96 hours significantly increased the activities of SOD and CAT, but significantly decreased GSH levels. Moreover, the transcription of Ucp-2 was significantly up-regulated to 4.12 folds(p<0.01)while that of Gstp2 and Nqo1 was significantly down-regulated to 0.62 folds and 0.55 folds(p<0.01), respectively, by 50μg·L-1 BDE-47. There was no significant difference observed in the expressions of Sod1 between the treatments and the control. Under exposure of 5, 10, and 50μg·L-1, olive tail moment increased significantly with increasing concentrations of ROS. These results indicate that BDE-47 could induce oxidative stress in zebrafish embryos and ROS is the trigger inducing embryos DNA damage.关键词
BDE-47/斑马鱼胚胎/氧化应激/DNA损伤Key words
BDE-47/zebrafish embryos/oxidative stress/DNA damage分类
资源环境引用本文复制引用
赵雪松,任新,杨春维,尤宏,任百祥..BDE-47对斑马鱼胚胎氧化应激与DNA损伤的毒性研究[J].农业环境科学学报,2015,(12):2280-2286,7.基金项目
四平市科技发展计划项目（四科合字第2014050号）；吉林师范大学2015年大学生创新创业计划项目 （）
