农业资源与环境学报Issue(6)：577-582,6.DOI:10.13254/j.jare.2015.0030
AA3型连续流动分析仪与钒钼黄比色法测定玉米植株全磷含量之比较
Determination Total Phosphour of Maize Plant Samples by Continuous Flow Analyzer in Comparison with Vanadium Molybdate Yellow Colorimetric Method
摘要
Abstract
The vanadium molybdate yellow colorimetric method(VMYC method)is regarded as one of conventional methods for determining total phosphorus(P)in plants, but it is time consuming procedure. Continuous flow analyzer(CFA)is a fluid stream segmentation technique with air segments. It is used to measure P concentration based on the molybdate-antimony-ascorbic acid method of Murphy and Riley. Sixty nine of maize plant samples were selected and digested with H2SO4-H2O2. P concentrations in the digests were determined by CFA and VMYC method, respectively. The t test found that there was no any significant difference of the plant P contents measured by the CFA and the VMYC method. A linear equation could best describe their relationship:Y(CFA-P)=0.927X(VMYC-P)-0.002. The Pearson’s correlation coefficient was 0.985 with a significance level(n=69, P<0.01). The CFA method for plant P measurement had a high precision with relative standard deviation(RSD)less than 1.5%. It is suggested that the CFA based on Murphy and Riley colorimetric detection can be used to determinate total plant P in the digests solutions with H2SO4-H2O2. The CFA method is labor saving and can handle large numbers of samples. The human error in mixing with other operations is reduced to a great extent.关键词
连续流动分析仪/钒钼黄比色法/植物全磷Key words
continuous flow analyzer(CFA)/vanadium molybdate yellow colorimetric method(VMYC method)/plant phosphorus分类
环境科学引用本文复制引用
刘云霞,温云杰,黄金莉,李桂花,柴晓,汪洪..AA3型连续流动分析仪与钒钼黄比色法测定玉米植株全磷含量之比较[J].农业资源与环境学报,2015,(6):577-582,6.基金项目
国家重点基础研究发展计划（“973”计划）项目（2013CB127402）；中央级公益性科研院所专项资金资助项目 （）
