山东农业科学Issue(12)：80-84,5.DOI:10.14083/j.issn.1001-4942.2015.12.020
山东部分地区棉花黄萎病菌的致病类型及毒性分析
Pathotypes and Virulence Analysis of Verticillium dahliae in Shandong Province
摘要
Abstract
Verticillium wilt is one of the most destructive diseases of cotton in Shandong Province.The samples of diseased cotton plants were collected from Dezou,Dongying and Taian,and 202 strains of Verticilli-um dahliae Kleb.were isolated and identified.The primers of INTD2f/INTD2r and INTND2f/INTND2r, which were specific for identifying defoliating (D)and non -defoliating (ND)pathotype respectively,were used to amplify the specific segment by PCR.At the same time,8 strains were randomly selected out to ana-lyze their toxin secretion ability and wilt index.The results showed that 201 strains were identified as D patho-type,and only 1 strain was identified as non -defoliating (ND)pathotype.The D pathotype of strain HDF4 -3 -1 showed the highest toxin secretion ability with the content of toxin as 50.82 μg/mL,however,the ND pathotype of strain HLX5 -1 -1 had lower toxin secretion ability with the toxin content as only 13.85 μg/mL. The wilt index of D pathotype of strain HDF5 -3 -1 was the highest as 72.5%,while that of ND pathotype of strain HLX5 -1 -1 was the lowest as 42.5%.In a word,D pathotype of strains had higher toxin secretion a-bility and wilt index,overall.关键词
棉花黄萎病菌/落叶型菌系/非落叶型菌系/产毒素能力/致萎指数Key words
Verticillium dahliae/Defoliating pathotype/Non -defoliating pathotype/Toxin secretion a-bility/Wilt index分类
农业科技引用本文复制引用
魏春芝,刘朝霞,武贵元,王志敏,沈法富,刘爱新..山东部分地区棉花黄萎病菌的致病类型及毒性分析[J].山东农业科学,2015,(12):80-84,5.基金项目
国家转基因重大专项 （）
