山东医药Issue(47)：7-9,3.DOI:10.3969/j.issn.1002-266X.2015.47.003
颞叶癫痫大鼠癫痫持续状态后海马组织中let-7e 和 miR-23的表达变化
Expression changes of let-7e and miR-23 in hippocampus tissues of rats with temporal lobe epilepsy after status epilepticus
摘要
Abstract
Objective To detect the expression changes of let-7e and miR-23 in the hippocampus tissues of rats with temporal lobe epilepsy ( TLE) at different time points after status epilepticus ( SE) .Methods A total of 160 rats were randomly divided into the control group ( n=10 ) which was injected with normal saline , and the TLE group ( n=150 ) which was induced by lithium-pilocarpine.TLE group was divided into 10 subgroups by different time points (0 hour, 1 hour, 6 hours, 12 hours, 1 day, 2 days, 7 days, 14 days, 30 days and 50 days) after SE and each subgroup was consist of 15 rats.The real-time fluorescent quantitative PCR was performed to detect the expression changes of let -7e and miR-23 in all rats.Results The relative expression levels of let-7e in the TLE subgroups were 0.61, 0.84, 0.85, 1.40, 10.74, 0.71, 0.65, 1.52, 1.69 and 0.13 at 0 h, 1 h, 6 h, 12 h, 1 d, 2 d, 7 d, 14 d, 30 d and 50 d.When we compared with that of the normal saline group, significant differences were found (all P<0.05).The relative expression levels of miR-23 in the TLE subgroups were 0.27, 0.31, 0.41, 0.39, 4.49, 0.21, 0.18, 0.12, 0.83 and 2.4 at 0 h, 1 h, 6 h, 12 h, 1 d, 2 d, 7 d, 14 d, 30 d and 50 d.When we compared with that of the normal saline group , significant differences were found (all P<0.05).Conclusions The expression of let-7e and miR-23 in the hippocampus of TLE rats was first up-reg-ulated and reached the peak value at 24 h after SE and then it began to down-regulate.A second up-regulated expression was seen from 10 d to 30 d after SE with let-7e and from 30 d to 50 d with miR-23.关键词
颞叶癫痫/癫痫持续状态/let-7e基因/miR-23基因/动物实验模型/海马Key words
temporal lobe epilepsy/status epilepticus/let-7e/mir-23 gene/experimental animal model/hippocampus分类
医药卫生引用本文复制引用
田小冰,程焱,宋毅军..颞叶癫痫大鼠癫痫持续状态后海马组织中let-7e 和 miR-23的表达变化[J].山东医药,2015,(47):7-9,3.基金项目
天津市应用基础及前沿技术研究计划（10JCYBJC13800）。 （）
