世界竹藤通讯Issue(6)：1-8,8.DOI:10.13640/j.cnki.wbr.2015.06.001
毛竹捕光色素结合蛋白基因结构及表达模式分析
Structure and Expression Analysis of Light-harvesting Chlorophyll a/b Binding Protein Genes in Moso Bamboo (Phyllostachys edulis)
摘要
Abstract
Plants convert the solar energy into chemical energy through photosynthesis, and the light-harvesting chloro-phyll a/b binding ( LHC) protein plays a crucial role in the process of energy capture, transfer and conversion. There-fore, the study on the structure and expression pattern of LHC genes is of great significance to reveal their functions in photosynthesis of moso bamboo ( Phyllostachys edulis) . A genome-wide analysis of LHC genes in moso bamboo was carried out using bioinformatics methods. The results showed that there were 29 LHC homologous genes in moso bam-boo, of which the intron numbers ranged from 0 to 5. On the basis of sequence analysis, the proteins encoded by 29 LHC genes belonged to LHCⅠ in PSⅠand LHCⅡ in PSⅡ respectively. LHCⅠ contained 5 subfamilies ( Lhca1-Lhca5), each of which only had one member except Lhca 4 with 3 members, while LHCⅡ contained 6 subfamilies (Lhcb1-Lhcb6) which have a varied range of members each, among which Lhcb1 subfamily had a maximum of seven members. Hydrophilic and hydrophobic prediction demonstrated that there was a certain difference in the members of different subfamilies. The results of protein structure prediction showed that all the 29 proteins encoded by LHC genes was composed of transit peptide and mature protein, which contained transmembrane structure and pigment binding sites. There were 12 proteins predicted with priority of alpha helix and 17 proteins with priority of random curl. The a-nalysis of gene expression profile suggested that most of LHC genes mainly express in leaves and panicles, and few in shoot, but almost undetectable in rhizome and root. Overall, these results laid a significant foundation for further study on the LHC gene function in moso bamboo.关键词
毛竹/LHC基因/结构/表达模式Key words
Moso bamboo/LHC gene/gene structure/expression pattern引用本文复制引用
李利超,孙化雨,娄永峰,赵韩生,高志民..毛竹捕光色素结合蛋白基因结构及表达模式分析[J].世界竹藤通讯,2015,(6):1-8,8.基金项目
国家自然科学基金“竹子叶黄素循环分子调节机制研究”(编号:31370588)；林业公益性行业科研专项“毛竹核心种质重测序及竹壁发育关键基因研究”(编号:201504106)。 （编号:31370588）
