中国生态农业学报Issue(1)：105-111,7.DOI:10.13930/j.cnki.cjea.150892
截形叶螨发育速率与温度关系模拟及对哒螨灵的抗性风险评估
Simulation of developmental rate and temperature trend, and assessement of resistance risk to pyridaben of Tetranychus truncatus Ehara
摘要
Abstract
In recent years, Tetranychus truncatus Ehara has become one of the main pests in Hexi area of Gansu Province, for which pyridaben was widely used due to its special physiological mechanism and broad-spectrum efficiency. However, T. truncatus has developed resistance to the sole application of pyridaben for a long perod of time. The aim of this paper was to verify the effect of temperature on risk development rate of resistant and susceptible populations of Tetranychus truncatus Ehara. Then based on the results of resistance selection, the study evaluated the resistance risk of T. truncatus to pyridaben. To do this, the relationship between development rate and temperature was analyzed using the Wang-Lan-Ding model at six temperatures (16℃, 20℃, 24℃, 28℃, 32℃and 36℃). The realized heritability (h2) of T. truncatus was estimated and the resistance risk of T. truncates to pyridaben under different resistance selection pressure predicted on the basis of resistance breeding and selection in the laboratory. Then threshold trait analysis was done in quantitative genetics to provide a theoretical support for the application of pyridaben and comprehensive control of T. truncatus. The results showed that based on the fitted Wang-Lan-Ding models, the minimum and maximum boundary temperatures of susceptible populations were 10.05 ℃ and 39.24 ℃, whereas that of the resistant populations were 13.45 ℃ and 41.89 ℃, respectively. The fitted models also showed that the maximum boundary temperature of resistant populations was significantly greater than that of susceptible populations. This implied that resistant populations had much stronger suitability to extreme temperatures than susceptible populations. The realized heritability (h2) of T. truncatus resistance to pyridaben was 0.11, and h2 for the first period and mid-term selection experiment (0.12 and 0.18, respectively) was higher than that for the later period (0.08), but h2 (0.14) sharply increased at terminal stage. Under laboratory conditions with h2= 0.11, developing a 10-fold increase of resistance to pyridaben required 10-23 generations under selection pressure (mortality) of 50%-90%. Under field conditions (h2= 0.05), it required 21-46 generations to develop the same resistance level.The results suggested that T. truncatus had resistance risk to pyridaben. However, when pyridaben was applied under rotation with other insecticides without cross-resistance and reduced selection pressure, the resistance development rate of T. truncatus delayed.关键词
截形叶螨/发育速率/温度/哒螨灵/抗性风险Key words
Tetranychus truncatus/Developmental rate/Temperature/Pyridaben/Resistance risk分类
农业科技引用本文复制引用
宋丽雯,沈慧敏..截形叶螨发育速率与温度关系模拟及对哒螨灵的抗性风险评估[J].中国生态农业学报,2016,(1):105-111,7.基金项目
公益性行业(农业)科研专项(201103020)、国家自然科学基金项目(31260442)和盛彤笙科技创新基金项目(GSAU-STS-1518)资助* Supported by the Special Fund for Agro-Scientific Research in the Public Interest (No.201103020), the National Natural Science Foundation of China (No.31260442) and the Sheng Tongsheng Innovation and Technology Fund (GSAU-STS-1518) （农业）
