生物技术通报Issue(12)：70-74,5.DOI:10.13560/j.cnki.biotech.bull.1985.2015.12.010
基于HUVEC细胞表面ELAM-1表达的抗TNF-α抗体活性检测方法
A Method for the Activity Assay of Anti-TNF-α Antibodies Expressed by ELAM-1 in HUVEC Surface
摘要
Abstract
This study is to develop a method to assay the biologic activity of anti-TNF-α(tumor necrosis factor-alpha)monoclonal antibodies. TNF-α stimulates the expression of adhesion molecules E-Selectin(ELAM-1)in human umbilical vein endothelial cells(HUVEC), whereas anti-TNF-αantibodies suppress the expression of ELAM-1 in HUVEC cells through neutralizing TNF-α. Based on this principle, we developed and validated a method for the bioactivity assay of the antibodies. The result showed that the assay method was established successfully, and the specificity of the method was validated to be feasible. The recovery rate was 92.1%-102.1%, and the RSD was≦ 7.66% while repeated 12 times. The linear range in 50% to 150% was fine, and the correlation coefficient was 0.99. Conclusively, this method is well suited as an activity assay of antibodiesin vitro.关键词
单克隆抗体/HUVEC细胞/ELAM-1/肿瘤坏死因子TNF-αKey words
monoclonal antibody/HUVEC cell/ELAM-1/TNF-α引用本文复制引用
陈坤,徐军,谢灿,周冬梅,杨彬,孙文正..基于HUVEC细胞表面ELAM-1表达的抗TNF-α抗体活性检测方法[J].生物技术通报,2015,(12):70-74,5.基金项目
广东省引进创新科研团队计划资助 （）
