生物技术通报Issue(12)：97-104,8.DOI:10.13560/j.cnki.biotech.bull.1985.2015.12.014
‘易丰’女贞组培快繁技术体系的建立
The Establishment of Tissue Culture and Rapid Propagation System of Ligustrum lucidum‘Yi Feng’
摘要
Abstract
Ligustrum lucidum‘Yi Feng’, a wild and variant species, because its resource was very scarce, and routine methods could not achieve large-scale propagation during a short period of time, a relatively perfect tissue culture and rapid propagation system for massive production was established in this research with axillary buds of young branch cuttings as explant materials. The results showed that, the optimal initial medium was WPM+ 0.3 mg/L KT + 0.1 mg/L 6-BA + 0.05 mg/LNAA, and the germination frequency of axillary buds reached 95.6%;the optimal proliferation medium was WPM+ 0.5 mg/L KT + 1.0 mg/L 6-BA + 0.05 mg/L NAA, and its average proliferation coefficient reached 5.63;the optimal rooting medium of shoots was 1/2WPM+ 1.5 mg/L IBA + 0.1 mg/L NAA. After about 20 days, the overall frequency of shoots rooting reached about 95.4%. And then, more than 95% of the plantlets could survive on the substrate soil in the end. The system can satisfy the need of industrial production, and the plantlets can steadily inherit the outstanding variation character of parents.关键词
‘易丰’女贞/快速繁殖/增殖培养Key words
Ligustrum lucidum‘Yi Feng’/rapid propagation/proliferation culture引用本文复制引用
孙英坤,代其林,张俊林,王劲,沈柏春,陈林敬,钱飞,吴光洪..‘易丰’女贞组培快繁技术体系的建立[J].生物技术通报,2015,(12):97-104,8.基金项目
国家重点基础研究发展计划（2013CB733903），国家高技术研究发展计划（2012AA063503），农业部公益性行业科研专项 （）
