生物技术通报Issue(12)：221-226,6.DOI:10.13560/j.cnki.biotech.bull.1985.2015.12.032
产D-乳酸重组大肠杆菌ptsG基因的敲除及其混合糖同步发酵
The Knockout of GeneptsG of Recombinant Escherichia coli Producing D-lactic Acid and the Simultaneous Fermentation of Mixed Sugars
摘要
Abstract
In order to construct a recombinant engineeringEscherichia colistrain that yields D-lactic acid in the simultaneous efficient fermentation of pentose and hexose, having an engineeringE. coli JH13 that efficiently ferment the pentose to produce D-lactic acid as an original strain, a glucose transmembrane transporter geneptsG was knocked outby the technique ofRed homologous recombination. The fermentative results showed that in the 10% mixed sugars(5% glucose and 5% xylose), theptsG-deleted strainE. coli JH15 simultaneously utilized pentose and hexose to complete the fermentation;however, the control strain started to utilize the xylose only after glucose was consumed up, and 18 g/L xylose still remained after the fermentation completed. The production of D-lactic acid by JH15 reached 83.04 g/L, and 25.86% higher than that by control strain JH13. The JH15 as aE. coli strain of producing D-lactic acid during simultaneous fermentation with mixed sugars, its construction provides a reference for producing the D-lactic acid in fermentation while utilizing the low-cost hydrolyzed components of lignocelluloses materials as raw material.关键词
重组大肠杆菌工程菌/D-乳酸/ptsG基因/Red同源重组/混合糖Key words
recombinant Escherichia coli/D-lactic acid/ptsG/Red homologous recombination/mixed sugars引用本文复制引用
丁小云,顾健健,王永泽,赵锦芳,王金华,赵筱..产D-乳酸重组大肠杆菌ptsG基因的敲除及其混合糖同步发酵[J].生物技术通报,2015,(12):221-226,6.基金项目
国家自然科学基金项目（NSFC31070094），湖北省自然科学基金项目（2011CDA008，2011CDB076），湖北工业大学博士科研启动基金项目 （）
